Development of a multi-locus sequence typing system helps reveal the evolution of Cardinium hertigii, a reproductive manipulator symbiont of insects

Table 2 MLST primers and their suggested melting temperatures for PCR

Primer name	Primer sequence (5′ – 3′)	Tm (°C)	Gene length (bp)	Amplified nucleotide range of gene (bp)	MLST fragment size (bp)
gyrb_859F	ATGCAYGTMACBGGDTTTARAAG	50	1950	859–1637	736
gyrb_1637R	TARAGTGGRGGRGARGCAAT	50	1950	859–1637	736
groel_346F	VTHAARCGBGGBATWGACAA	52	1638	346–842	476
groel_287F^a	CNCARKCTATWTTYRYVCATGG
groel_842R	TTGGBGAYAGAAGRAARGCNATG
sufb_806F	CTACNGTDCARAATTGGTATCC	50	1443	806–1289	451
sufb_1289R	ADYTGRTCYKCRCTRATTTT	50	1443	806–1289	451
EF_1689R	AAABCCYTTYTGAATIGCTGG	52	2142	1689–1162	482
EF_1162F	GCNGTRGTIGGITTTAARGARATTA	52	2142	1689–1162	482

ISSN: 1471-2180