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Table 2 MLST primers and their suggested melting temperatures for PCR

From: Development of a multi-locus sequence typing system helps reveal the evolution of Cardinium hertigii, a reproductive manipulator symbiont of insects

Primer name Primer sequence (5′ – 3′) Tm (°C) Gene length (bp) Amplified nucleotide range of gene (bp) MLST fragment size (bp)
gyrb_859F ATGCAYGTMACBGGDTTTARAAG 50 1950 859–1637 736
gyrb_1637R TARAGTGGRGGRGARGCAAT
groel_346F VTHAARCGBGGBATWGACAA 52 1638 346–842 476
groel_287Fa CNCARKCTATWTTYRYVCATGG
groel_842R TTGGBGAYAGAAGRAARGCNATG
sufb_806F CTACNGTDCARAATTGGTATCC 50 1443 806–1289 451
sufb_1289R ADYTGRTCYKCRCTRATTTT
EF_1689R AAABCCYTTYTGAATIGCTGG 52 2142 1689–1162 482
EF_1162F GCNGTRGTIGGITTTAARGARATTA
  1. aAlternative forward primer for groEl