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Table 2 MLST primers and their suggested melting temperatures for PCR

From: Development of a multi-locus sequence typing system helps reveal the evolution of Cardinium hertigii, a reproductive manipulator symbiont of insects

Primer name

Primer sequence (5′ – 3′)

Tm (°C)

Gene length (bp)

Amplified nucleotide range of gene (bp)

MLST fragment size (bp)

gyrb_859F

ATGCAYGTMACBGGDTTTARAAG

50

1950

859–1637

736

gyrb_1637R

TARAGTGGRGGRGARGCAAT

groel_346F

VTHAARCGBGGBATWGACAA

52

1638

346–842

476

groel_287Fa

CNCARKCTATWTTYRYVCATGG

groel_842R

TTGGBGAYAGAAGRAARGCNATG

sufb_806F

CTACNGTDCARAATTGGTATCC

50

1443

806–1289

451

sufb_1289R

ADYTGRTCYKCRCTRATTTT

EF_1689R

AAABCCYTTYTGAATIGCTGG

52

2142

1689–1162

482

EF_1162F

GCNGTRGTIGGITTTAARGARATTA

  1. aAlternative forward primer for groEl