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Table 2 MLST primers and their suggested melting temperatures for PCR

From: Development of a multi-locus sequence typing system helps reveal the evolution of Cardinium hertigii, a reproductive manipulator symbiont of insects

Primer namePrimer sequence (5′ – 3′)Tm (°C)Gene length (bp)Amplified nucleotide range of gene (bp)MLST fragment size (bp)
gyrb_859FATGCAYGTMACBGGDTTTARAAG501950859–1637736
gyrb_1637RTARAGTGGRGGRGARGCAAT
groel_346FVTHAARCGBGGBATWGACAA521638346–842476
groel_287FaCNCARKCTATWTTYRYVCATGG
groel_842RTTGGBGAYAGAAGRAARGCNATG
sufb_806FCTACNGTDCARAATTGGTATCC501443806–1289451
sufb_1289RADYTGRTCYKCRCTRATTTT
EF_1689RAAABCCYTTYTGAATIGCTGG5221421689–1162482
EF_1162FGCNGTRGTIGGITTTAARGARATTA
  1. aAlternative forward primer for groEl
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