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Fig. 4 | BMC Microbiology

Fig. 4

From: A mouse ear skin model to study the dynamics of innate immune responses against Staphylococcus aureus biofilms

Fig. 4

Motility of recruited EGFP+ cells in the mouse ear pinna after micro-injection of Staphylococcus aureus. a and b Illustration of immune cell tracking with Imaris software using the “Spots” tool to analyse the motility of recruited immune cells. The analysis was carried out in different zones of the injection site where cells were either in contact with visible bacteria (a) or not (b). Each cell is represented by a white sphere and its trajectory in the thickness of the tissue by a multicoloured line. Images shown were taken at 4.45 hpi (a) and 26 hpi (b). *: base of hair follicles. Scale bar: 50 μm. c–h Average speed and straightness of EGFP+ cells recruited to injection sites at early and late time points after inoculation of TS culture medium (control), planktonic bacteria (planktonic form) or biofilms (biofilm form). Data are expressed as median and interquartile ranges pooled from three different mice in three independent experiments for each group. Average speed (c) and straightness (d) of all cells (in contact with visible bacteria or not) in infected and control mice. Number of cells (N) analysed for each group at early and late time points, respectively: Control: N = 90 and 94 cells; Planktonic form: N = 315 and 433 cells; Biofilm form: N = 254 and 518 cells. Average speed (e and g) and straightness (f and h) of cells either in contact (bacteria contact) or not (no bacteria contact) with planktonic or biofilm bacteria at early (e and f) and late (g and h) time points. Number of cells (N) analysed at early time points that were in contact or not in contact with bacteria, respectively: Planktonic form: N = 157 and 158 cells; Biofilm form: N = 142 and 112 cells. Number of cells (N) analysed at late time points that were in contact or not in contact with bacteria, respectively: Planktonic form: N = 298 and 135 cells; Biofilm form: N = 98 and 420 cells

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