Fig. 1

L. monocytogenes expresses caseinolytic active HtrA. Equal amounts of protein lysates of L. monocytogenes 10403 s, L. monocytogenes ∆htrA, and L. monocytogenes EGD were analyzed by casein zymography (left panel) to detect caseinolytic active proteases and Western blotting (right panel) to detect HtrA expression. The zymograms have been repeated four times and the HtrA detection by Western blotting has been repeated at least three times using lysates from independent experiments