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Table 2 Characteristics of whole genome datasets of selective B. cereus s.l. strains

From: Antibiotics resistance and toxin profiles of Bacillus cereus-group isolates from fresh vegetables from German retail markets

Characteristic Bacillus cereus s.l. isolate
B26 G12 MS12 MS17 MS195 MS464a MS532a MS735
No. of contigs 67 64 368 48 79 41 75 77
Largest contig 524,166 855,854 262,491 733,489 594,878 746,015 1,014,035 657,340
N50 228,834 193,941 44,422 251,250 146,479 249,433 154,418 201,858
GC-content (mol%) 35.27 35.08 34.73 35.31 34.90 35.27 34.70 34.89
Total length
(bp)
5,439,456 5,686,136 6,345,988 5,472,616 6,018,360 5,170,399 6,113,939 5,964,825
MLST (B. cereus) ST-352 unknown ST-15 unknown ST-278 ST-163 unknown ST-72
Species (KmerFinder) Bacillus weihenstephanensis Bacillus cereus Bacillus thuringiensis Bacillus weihenstephanensis Bacillus toyonensis Bacillus cereus Bacillus thuringiensis Bacillus toyonensis
Antibiotic resistance
phenotypeA
AMP, CTX, AMG, PEN, SXT, GM AMP, CTX, AMG, PEN, SXT AMP, CTX, AMG, PEN AMP, CTX, AMG, PEN, SXT AMP, CTX, AMG, PEN, ERY, AMP, CTX, AMG, PEN, SXT, GEN AMP, CTX, AMG, PEN, TET, GEN, ERY, CHL AMP, CTX, AMG, PEN, ERY,
Enterotoxin PCR nhe+, hbl+, cytK, ces nhe+, hbl+, cytK+, ces nhe-, hbl+, cytK+, ces nhe+, hbl, cytK, ces nhe+, hbl+, cytK, ces nhe+, hbl, cytK, ces nhe+, hbl+, cytK+, ces nhe+, hbl+, cytK, ces
Enterotoxin serology Nhe+, Hbl Nhe+, Hbl+ Nhe+, Hbl+ Nhe+, Hbl Nhe+, Hbl+ Nhe+, Hbl Nhe+, Hbl+ Nhe+, Hbl+
Enterotoxin genes identified on genome nhe and hbl genes nhe, hbl and cytK genes nhe, hbl and cytK genes nhe and hbl genes nhe and hbl genes nhe and hbl genes nhe, hbl and cytK genes nhe and hbl genes
  1. Species identification was carried out with KmerFinder (PATRIC) which examined the number of co-occurring k-mers (substrings of k nucleotides in genome sequence data) and MLST was done by PATRIC to assign the strains to clonal lineages. AbbreviationsA: amikacin (AMK), ampicillin (AMP), amoxicillin/clavulanic acid (AMG), cefotaxime (CTX), chloramphenicol (CHL), ciprofloxacin (CIP), erythromycin (ERY), gentamicin (GEN), imipenem (IPM), penicillin G (PEN), tetracycline (TET) and trimethoprim-sulfamethoxazole (SXT)