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Table 1 Incidences of toxin genes hbl, nhe, cytK-2 and ces detection and of toxins Nhe and Hbl expression

From: Antibiotics resistance and toxin profiles of Bacillus cereus-group isolates from fresh vegetables from German retail markets

Department/laboratoryPresence of toxin gene detected in multiplex PCRToxin gene profile (classification according to Ehling-Schulz et al. [16])Duopath® test for toxin production
hblDnheAcytK-2ceshbl+/nhe+/
cytK+/ces-
(A)
hbl+/nhe+/
cytK−/ces-
(C)
hbl+/nhe−/
cytK+/ces-
-
hbl−/nhe+/
cytK−/ces-
(F)
hbl−/nhe-cytK+/ces-
(G)
NheHbl
Microbiology and Biotechnology (Kiel)
Laboratory 1
134/147 (91.2%)108/147 (73.5%)55/147 (37.4%)0/147 (0%)16/147 (10.9%)79/147 (53.7%)39/147 (26.5%)13/147 (8.8%)0/147 (0%)122/147 (83.0%)93/147 (63.3%)
Safety and Quality of Fruit and Vegetables (Karlsruhe) Laboratory 2128/147 (87.1%)107/147 (72.8%)55/147 (37.4%)0/147 (0%)15/147 (10.2%)78/147 (53.1%)35/147 (23.8%)14/147 (9.5%)5/147 (3.4%)
Combined result of multiplex PCR and Duopath® test for toxin production
Microbiology and Biotechnology (Kiel)
Laboratory 1
    55/147 (37.4%)79/147 (53.7%)0/147 (0%)13/147 (8.8%)0/147 (0%)
Safety and Quality of Fruit and Vegetables (Karlsruhe) Laboratory 2    55/147 (37.4%)79/147 (53.7%)0/147 (0%)13/147 (8.8%)0/147 (0%)