Table 1 Incidences of toxin genes hbl, nhe, cytK-2 and ces detection and of toxins Nhe and Hbl expression
| Department/laboratory | Presence of toxin gene detected in multiplex PCR | Toxin gene profile (classification according to Ehling-Schulz et al. [16]) | Duopath® test for toxin production | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| hblD | nheA | cytK-2 | ces |
hbl+/nhe+/ cytK+/ces- (A) |
hbl+/nhe+/ cytK−/ces- (C) |
hbl+/nhe−/ cytK+/ces- - |
hbl−/nhe+/ cytK−/ces- (F) |
hbl−/nhe-cytK+/ces- (G) | Nhe | Hbl | |
|
Microbiology and Biotechnology (Kiel) Laboratory 1 | 134/147 (91.2%) | 108/147 (73.5%) | 55/147 (37.4%) | 0/147 (0%) | 16/147 (10.9%) | 79/147 (53.7%) | 39/147 (26.5%) | 13/147 (8.8%) | 0/147 (0%) | 122/147 (83.0%) | 93/147 (63.3%) |
| Safety and Quality of Fruit and Vegetables (Karlsruhe) Laboratory 2 | 128/147 (87.1%) | 107/147 (72.8%) | 55/147 (37.4%) | 0/147 (0%) | 15/147 (10.2%) | 78/147 (53.1%) | 35/147 (23.8%) | 14/147 (9.5%) | 5/147 (3.4%) | ||
| Combined result of multiplex PCR and Duopath® test for toxin production | |||||||||||
|
Microbiology and Biotechnology (Kiel) Laboratory 1 | 55/147 (37.4%) | 79/147 (53.7%) | 0/147 (0%) | 13/147 (8.8%) | 0/147 (0%) | ||||||
| Safety and Quality of Fruit and Vegetables (Karlsruhe) Laboratory 2 | 55/147 (37.4%) | 79/147 (53.7%) | 0/147 (0%) | 13/147 (8.8%) | 0/147 (0%) | ||||||