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Table 1 Incidences of toxin genes hbl, nhe, cytK-2 and ces detection and of toxins Nhe and Hbl expression

From: Antibiotics resistance and toxin profiles of Bacillus cereus-group isolates from fresh vegetables from German retail markets

Department/laboratory Presence of toxin gene detected in multiplex PCR Toxin gene profile (classification according to Ehling-Schulz et al. [16]) Duopath® test for toxin production
hblD nheA cytK-2 ces hbl+/nhe+/
cytK+/ces-
(A)
hbl+/nhe+/
cytK−/ces-
(C)
hbl+/nhe−/
cytK+/ces-
-
hbl−/nhe+/
cytK−/ces-
(F)
hbl−/nhe-cytK+/ces-
(G)
Nhe Hbl
Microbiology and Biotechnology (Kiel)
Laboratory 1
134/147 (91.2%) 108/147 (73.5%) 55/147 (37.4%) 0/147 (0%) 16/147 (10.9%) 79/147 (53.7%) 39/147 (26.5%) 13/147 (8.8%) 0/147 (0%) 122/147 (83.0%) 93/147 (63.3%)
Safety and Quality of Fruit and Vegetables (Karlsruhe) Laboratory 2 128/147 (87.1%) 107/147 (72.8%) 55/147 (37.4%) 0/147 (0%) 15/147 (10.2%) 78/147 (53.1%) 35/147 (23.8%) 14/147 (9.5%) 5/147 (3.4%)
Combined result of multiplex PCR and Duopath® test for toxin production
Microbiology and Biotechnology (Kiel)
Laboratory 1
     55/147 (37.4%) 79/147 (53.7%) 0/147 (0%) 13/147 (8.8%) 0/147 (0%)
Safety and Quality of Fruit and Vegetables (Karlsruhe) Laboratory 2      55/147 (37.4%) 79/147 (53.7%) 0/147 (0%) 13/147 (8.8%) 0/147 (0%)