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Table 1 Incidences of toxin genes hbl, nhe, cytK-2 and ces detection and of toxins Nhe and Hbl expression

From: Antibiotics resistance and toxin profiles of Bacillus cereus-group isolates from fresh vegetables from German retail markets

Department/laboratory

Presence of toxin gene detected in multiplex PCR

Toxin gene profile (classification according to Ehling-Schulz et al. [16])

Duopath® test for toxin production

hblD

nheA

cytK-2

ces

hbl+/nhe+/

cytK+/ces-

(A)

hbl+/nhe+/

cytK−/ces-

(C)

hbl+/nhe−/

cytK+/ces-

-

hbl−/nhe+/

cytK−/ces-

(F)

hbl−/nhe-cytK+/ces-

(G)

Nhe

Hbl

Microbiology and Biotechnology (Kiel)

Laboratory 1

134/147 (91.2%)

108/147 (73.5%)

55/147 (37.4%)

0/147 (0%)

16/147 (10.9%)

79/147 (53.7%)

39/147 (26.5%)

13/147 (8.8%)

0/147 (0%)

122/147 (83.0%)

93/147 (63.3%)

Safety and Quality of Fruit and Vegetables (Karlsruhe) Laboratory 2

128/147 (87.1%)

107/147 (72.8%)

55/147 (37.4%)

0/147 (0%)

15/147 (10.2%)

78/147 (53.1%)

35/147 (23.8%)

14/147 (9.5%)

5/147 (3.4%)

Combined result of multiplex PCR and Duopath® test for toxin production

Microbiology and Biotechnology (Kiel)

Laboratory 1

    

55/147 (37.4%)

79/147 (53.7%)

0/147 (0%)

13/147 (8.8%)

0/147 (0%)

Safety and Quality of Fruit and Vegetables (Karlsruhe) Laboratory 2

    

55/147 (37.4%)

79/147 (53.7%)

0/147 (0%)

13/147 (8.8%)

0/147 (0%)