Antimicrobial activities and in vitro properties of cold-adapted Lactobacillus strains isolated from the intestinal tract of cold water fishes of high latitude water areas in Xinjiang, China

Table 3 Primers and PCR conditions used in rep-PCR

Method	Primer	Sequence (5′-3′)	PCR conditions
Method	Primer	Sequence (5′-3′)	Denaturation	Annealing	Extension
BOX-PCR	BOXA 1R	CTACGGCAAGGCGACGCTGACG	94 °C, 1 min	53 °C, 1 min	65 °C, 8 min
(GTG)₅-PCR	(GTG)₅	GTGGTGGTGGTGGTG	94 °C, 30 s	50 °C, 30 s	65 °C, 8 min

ISSN: 1471-2180