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Table 3 Primers and PCR conditions used in rep-PCR

From: Antimicrobial activities and in vitro properties of cold-adapted Lactobacillus strains isolated from the intestinal tract of cold water fishes of high latitude water areas in Xinjiang, China

MethodPrimerSequence (5′-3′)PCR conditions
BOX-PCRBOXA 1RCTACGGCAAGGCGACGCTGACG94 °C, 1 min53 °C, 1 min65 °C, 8 min
(GTG)5-PCR(GTG)5GTGGTGGTGGTGGTG94 °C, 30 s50 °C, 30 s65 °C, 8 min