Skip to main content

Table 3 The metabolites content (μg/g DCW) responsible for responding to fluconazole in Schizochytrium

From: The role of fluconazole in the regulation of fatty acid and unsaponifiable matter biosynthesis in Schizochytrium sp. MYA 1381

Metabolites 72 h 96 h 120 h
control fluconazole control fluconazole control fluconazole
TCA cycle
 Oxalic acid 924.4 ± 22.7 557.3 ± 18.6* 1317.5 ± 114.3 597.8 ± 122.1* 575.9 ± 48.1 737.7 ± 33.3*
 Butanedioic acid 21.4 ± 7.1 24.1 ± 11.1 11.3 ± 1.6 10.9 ± 2.4 21.1 ± 6.6 18.1 ± 5.3
 Citric acid 22.6 ± 0.6 8.3 ± 1.1* 22.6 ± 9.9 13.6 ± 6.9* 26.9 ± 7.8 22.6 ± 8.3*
Glycolysis pathway
 Glucose 8503.2 ± 591.9 6838.7 ± 180.3* 654.1 ± 10.4 904.9 ± 18.4 154.2 ± 69.3 118.9 ± 48.3
 Glycerol 633.5 ± 82.5 469.4 ± 54.5* 351.3 ± 14.9 226.7 ± 27.8* 227.5 ± 17.8 399.7 ± 1111.9*
 Ethenal 75.7 ± 4.9 51.7 ± 9.8* 33.0 ± 0.7 25.3 ± 10.3* 163.3 ± 29.2 154.7 ± 31.5
 Phosphate acid 12,811.2 ± 324.3 8389.3 ± 701.5 4413.8 ± 511.0 4622.4 ± 119.0* 10,915.5 ± 2687.4 9960.5 ± 4480.4
Amino acids
 Glycine 74.9 ± 11.6 74.1 ± 11.1* 234.5 ± 23.8 148.5 ± 9.8* 147.4 ± 70.0 163.9 ± 63.2
 L-Serine 12.2 ± 3.2 10.1 ± 5.0 22.1 ± 1.1 18.6 ± 1.4* 4.4 ± 4.4 18.4 ± 8.2
 β-Alanine 0.1 ± 0.0 0.3 ± 0.0* 7.4 ± 0.8 9.0 ± 1.4* 17.1 ± 4.4 29.8 ± 8.6*
 Proline 225.0 ± 28.2 202.7 ± 22.8* 156.1 ± 55.1 322.9 ± 75.2* 427.1 ± 123.8 337.8 ± 40.7
 L-Glutamic acid 7.1 ± 0.4 8.2 ± 1.9 10.2 ± 1.6 6.3 ± 5.0* 9.4 ± 1.6 8.9 ± 1.5
 Phenylalanine 13.7 ± 0.7 29.0 ± 5.3* 10.8 ± 4.6 6.0 ± 4.5* 21.1 ± 2.6 14.6 ± 4.0
Pentose phosphate pathway
 Xylose 7.8 ± 1.0 5.6 ± 0.5* 10.6 ± 1.2 7.1 ± 11.4* 17.8 ± 3.5 22.5 ± 3.8
 Ribitol 30.7 ± 1.2 20.8 ± 2.9* 54.1 ± 0.5 44.7 ± 23.4* 18.1 ± 5.3 20.9 ± 3.0
 Trehalose 21.1 ± 7.7 11.9 ± 11.1* 42.6 ± 3.7 59.8 ± 6.1* 45.5 ± 5.0 69.2 ± 6.4
Fatty acids
 Monostearate 23.5 ± 16.1 21.5 ± 10.5* 127.2 ± 18.5 239.5 ± 48.2* 155.9 ± 29.0 242.6 ± 31.0*
 Monopalmitin 17.7 ± 2.3* 17.6 ± 4.6 57.1 ± 9.0 83.2 ± 6.8* 268.0 ± 47.3 375.8 ± 60.3*
 Palmitic acid 1166.0 ± 77.5 889.9 ± 119.5 1712.2 ± 113.8 1262.7 ± 86.2* 1188.5 ± 151.0 1224.8 ± 246.0
 Stearic acid 1018.4 ± 107.2 648.4 ± 157.5* 593.5 ± 82.7 748.5 ± 72.6 703.9 ± 113.1 763.0 ± 109.9
 Arachidonic acid 19.9 ± 4.1 33.8 ± 4.6* 28.5 ± 2.5 36.3 ± 2.4* 41.9 ± 4.1 61.5 ± 15.2*
 Doconexent acid 237.5 ± 32.4 359.0 ± 26.9* 314.2 ± 33.6 267.0 ± 17.4 169.7 ± 31.5 271.5 ± 34.6*
Sterols
 Squalene 510.2 ± 33.8 610.6 ± 80.0* 161.4 ± 5.8 63.1 ± 0.3* 88.6 ± 10.7 16.8 ± 8.5*
 Cholesterol 271.4 ± 40.9 202.9 ± 10.2* 246.8 ± 18.0 95.8 ± 2.9* 286.8 ± 28.3 254.2 ± 20.5
 Cyclo-ergosta 120.8 ± 6.3 111.6 ± 7.9* 194.5 ± 18.8 204.4 ± 12.0 198.2 ± 28.9 287.5 ± 80.2*
 β-Sitosterol 10.9 ± 2.5 47.8 ± 3.4* 86.3 ± 18.8 81.8 ± 4.7 51.1 ± 4.8 122.9 ± 32.4*
 Cholest-7-en-3-ol 8.2 ± 0.1 0.6 ± 0.3* 51.0 ± 4.5 6.0 ± 0.4* 48.2 ± 7.0 8.4 ± 2.0*
 Cholecalciferol 7.1 ± 0.4 2.2 ± 0.5* 30.5 ± 4.7 10.4 ± 0.3* 32.7 ± 9.7 16.1 ± 8.2*
 Stigmasterol 15.0 ± 6.4 353.7 ± 29.9* 14.7 ± 3.7 483.9 ± 9.9* 19.9 ± 13.8 638.1 ± 108.3*
 Lanosterol 47.0 ± 4.8 152.9 ± 2.9* 9.6 ± 0.7 37.9 ± 1.0* 9.2 ± 1.6 72.2 ± 25.7*
Others
 Urea 2103.6 ± 183.8 1248.0 ± 130.2* 76.2 ± 4.2 32.9 ± 18.6* 71.4 ± 13.2 59.5 ± 19.9
  1. * p < 0.05
  2. The data represents the contents of metabolites and are showed as the mean ± SD. All data are four of replicates