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Table 3 The metabolites content (μg/g DCW) responsible for responding to fluconazole in Schizochytrium

From: The role of fluconazole in the regulation of fatty acid and unsaponifiable matter biosynthesis in Schizochytrium sp. MYA 1381

Metabolites72 h96 h120 h
controlfluconazolecontrolfluconazolecontrolfluconazole
TCA cycle
 Oxalic acid924.4 ± 22.7557.3 ± 18.6*1317.5 ± 114.3597.8 ± 122.1*575.9 ± 48.1737.7 ± 33.3*
 Butanedioic acid21.4 ± 7.124.1 ± 11.111.3 ± 1.610.9 ± 2.421.1 ± 6.618.1 ± 5.3
 Citric acid22.6 ± 0.68.3 ± 1.1*22.6 ± 9.913.6 ± 6.9*26.9 ± 7.822.6 ± 8.3*
Glycolysis pathway
 Glucose8503.2 ± 591.96838.7 ± 180.3*654.1 ± 10.4904.9 ± 18.4154.2 ± 69.3118.9 ± 48.3
 Glycerol633.5 ± 82.5469.4 ± 54.5*351.3 ± 14.9226.7 ± 27.8*227.5 ± 17.8399.7 ± 1111.9*
 Ethenal75.7 ± 4.951.7 ± 9.8*33.0 ± 0.725.3 ± 10.3*163.3 ± 29.2154.7 ± 31.5
 Phosphate acid12,811.2 ± 324.38389.3 ± 701.54413.8 ± 511.04622.4 ± 119.0*10,915.5 ± 2687.49960.5 ± 4480.4
Amino acids
 Glycine74.9 ± 11.674.1 ± 11.1*234.5 ± 23.8148.5 ± 9.8*147.4 ± 70.0163.9 ± 63.2
 L-Serine12.2 ± 3.210.1 ± 5.022.1 ± 1.118.6 ± 1.4*4.4 ± 4.418.4 ± 8.2
 β-Alanine0.1 ± 0.00.3 ± 0.0*7.4 ± 0.89.0 ± 1.4*17.1 ± 4.429.8 ± 8.6*
 Proline225.0 ± 28.2202.7 ± 22.8*156.1 ± 55.1322.9 ± 75.2*427.1 ± 123.8337.8 ± 40.7
 L-Glutamic acid7.1 ± 0.48.2 ± 1.910.2 ± 1.66.3 ± 5.0*9.4 ± 1.68.9 ± 1.5
 Phenylalanine13.7 ± 0.729.0 ± 5.3*10.8 ± 4.66.0 ± 4.5*21.1 ± 2.614.6 ± 4.0
Pentose phosphate pathway
 Xylose7.8 ± 1.05.6 ± 0.5*10.6 ± 1.27.1 ± 11.4*17.8 ± 3.522.5 ± 3.8
 Ribitol30.7 ± 1.220.8 ± 2.9*54.1 ± 0.544.7 ± 23.4*18.1 ± 5.320.9 ± 3.0
 Trehalose21.1 ± 7.711.9 ± 11.1*42.6 ± 3.759.8 ± 6.1*45.5 ± 5.069.2 ± 6.4
Fatty acids
 Monostearate23.5 ± 16.121.5 ± 10.5*127.2 ± 18.5239.5 ± 48.2*155.9 ± 29.0242.6 ± 31.0*
 Monopalmitin17.7 ± 2.3*17.6 ± 4.657.1 ± 9.083.2 ± 6.8*268.0 ± 47.3375.8 ± 60.3*
 Palmitic acid1166.0 ± 77.5889.9 ± 119.51712.2 ± 113.81262.7 ± 86.2*1188.5 ± 151.01224.8 ± 246.0
 Stearic acid1018.4 ± 107.2648.4 ± 157.5*593.5 ± 82.7748.5 ± 72.6703.9 ± 113.1763.0 ± 109.9
 Arachidonic acid19.9 ± 4.133.8 ± 4.6*28.5 ± 2.536.3 ± 2.4*41.9 ± 4.161.5 ± 15.2*
 Doconexent acid237.5 ± 32.4359.0 ± 26.9*314.2 ± 33.6267.0 ± 17.4169.7 ± 31.5271.5 ± 34.6*
Sterols
 Squalene510.2 ± 33.8610.6 ± 80.0*161.4 ± 5.863.1 ± 0.3*88.6 ± 10.716.8 ± 8.5*
 Cholesterol271.4 ± 40.9202.9 ± 10.2*246.8 ± 18.095.8 ± 2.9*286.8 ± 28.3254.2 ± 20.5
 Cyclo-ergosta120.8 ± 6.3111.6 ± 7.9*194.5 ± 18.8204.4 ± 12.0198.2 ± 28.9287.5 ± 80.2*
 β-Sitosterol10.9 ± 2.547.8 ± 3.4*86.3 ± 18.881.8 ± 4.751.1 ± 4.8122.9 ± 32.4*
 Cholest-7-en-3-ol8.2 ± 0.10.6 ± 0.3*51.0 ± 4.56.0 ± 0.4*48.2 ± 7.08.4 ± 2.0*
 Cholecalciferol7.1 ± 0.42.2 ± 0.5*30.5 ± 4.710.4 ± 0.3*32.7 ± 9.716.1 ± 8.2*
 Stigmasterol15.0 ± 6.4353.7 ± 29.9*14.7 ± 3.7483.9 ± 9.9*19.9 ± 13.8638.1 ± 108.3*
 Lanosterol47.0 ± 4.8152.9 ± 2.9*9.6 ± 0.737.9 ± 1.0*9.2 ± 1.672.2 ± 25.7*
Others
 Urea2103.6 ± 183.81248.0 ± 130.2*76.2 ± 4.232.9 ± 18.6*71.4 ± 13.259.5 ± 19.9
  1. * p < 0.05
  2. The data represents the contents of metabolites and are showed as the mean ± SD. All data are four of replicates