Skip to main content
Fig. 3 | BMC Microbiology

Fig. 3

From: The role of Acinetobacter baumannii response regulator BfmR in pellicle formation and competitiveness via contact-dependent inhibition system

Fig. 3

A. baumannii V15 contains a functional CDI system. a Multiple amino acid sequence alignment of each of the CDIV15 loci encoded proteins with the most similar type-I CDI system (bau-A1/Genbank accession number: AMFH01000034.1). The most similar CDI was determined by performing multiple sequence comparisons with the recently characterized Acinetobacter CDI systems [28]. Alignments are displayed as sequence fingerprints using alignment shading software Texshade (version 1.25) [29]. Identical amino acids are shaded black. Unique residues are shaded gray. Annotations above alignments indicate the aligned CDI proteins. b Expression of A. baumannii cdiB in WT, ΔbfmRS, and ΔbfmRS mutant complemented with pbfmR. The data are displayed as a fold change compared to the WT which is set at 1. c and d Part of 10% SDS-PAGE gel view showing the presence of the CdiA protein found in the total protein fraction from culture media of WT, ΔcdiV15, ΔbfmRS, ΔbfmRSΔcdiV15, and ΔbfmRS and ΔbfmRSΔcdiV15 complemented with pbfmR. Proteins were precipitated with TCA, separated by 10% SDS-PAGE, and visualized by staining with Coomassie blue. Numbers on the left denote molecular mass in kDa. e Quantitative evaluation of inter-bacterial competition assay displaying a recovered number of A. baylyi ADP1 with or without plasmid pcdiIV15 containing A. baumannii V15 immunity gene cdiI. Competition was performed with A. baumannii V15 mutants ΔbfmRS, ΔbfmRSΔhcp, ΔbfmRSΔcdiV15, ΔbfmRSΔhcpΔcdiV15 as the aggressors. E. coli DH5α was used as a negative non-competitive control to enumerate bacteria numbers if there were no competition. Error bars represent standard deviation. The horizontal lines represent mean value. Values were calculated from at least three independent experiments. *, p < 0.05; ***, p < 0.001; n.s., not significant. cdiIV15 gene in plasmid pcdiIV15 was induced using IPTG concentration of 5 mM

Back to article page