Prevalence and antibiotic susceptibility patterns of enteric bacterial pathogens in human and non-human sources in an urban informal settlement in Cape Town, South Africa

Table 2 Primers for amplification of diarrheic E. coli virulence genes by conventional PCR

	Target genes	Primers	Primer sequence	Product size	Reference
A	Eae	eae-F	TCAATGCAGTTCCGTTATCAGTT	482 bp	[20, 21]
	Eae	eae-R	GTAAAGTCCGTTACCCCAACCTG
	Bfp	bfp-F	GGAAGTCAAATTCATGGGGGTAT	298 bp	[21]
	Bfp	bfp-R	GGAATCAGACGCAGACTGGTAGT
	stx ₁	stx₁-F	CAGTTAATGTGGTGGCGAAGG	348 bp	[22]
	stx ₁	stx₁-R	CACCAGACAATGTAACCGCTG
	stx ₂	stx₂-F	ATCCTATTCCCGGGAGTTTACG	584 bp	[22]
	stx ₂	stx₂-R	GCGTCATCGTATACACAGGAGC
B	Est	ST-F	ATTTTTCTTTCTGTATTGTCTT	190 bp	[19]
	Est	ST-R	CACCCGGTACAAGCAGGATT
	Elt	LT-F	GGCGACAGATTATACCGTGC	440 bp	[19]
	Elt	LT-R	CGGTCTCTATATTCCCTGTT
C	Ipa	ipaH-F	CTCGGCACGTTTTAATAGTCTGG	933 bp	[21]
	Ipa	ipaH-R	GTGGAGAGCTGAAGTTTCTCTGC
	Aat	pCVD432-F	CTGGCGAAAGACTGTATCAT	630 bp	[19]
	Aat	pCVD432-R	CAATGTATAGAAATCCGCTGTT
	daaC	daaC-F	CAGGTCATCCGGTCAGTCGG	212 bp	[19]
	daaC	daaC-R	CAATGCCACGTACAACCGGC

ISSN: 1471-2180