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Table 2 The broth acidification rate by novel LAB strains isolated from silage growing in wells with 48-h old biofilms of pathogenic bacteria

From: Newly isolated lactic acid bacteria from silage targeting biofilms of foodborne pathogens during milk fermentation

 GlucoseStrainsL. plantarum
AG 1
P. acidilactici
AG 8
L. plantarum
AG 9
L. plantarum
AG 10
L. plantarum
AG 15
L. fermentum
AG 16
L. plantarum
8PA3
ΔpH2%LAB monoculture3.4 ± 0.35*3.2 ± 0.24*3.4 ± 0.31*3.6 ± 0.56*3.3 ± 0.23*3.2 ± 0.11*2.3 ± 0.15
P. aeruginosa2.5 ± 0.552.6 ± 0.22*2.5 ± 0.542.5 ± 0.52.4 ± 0.393.1 ± 0.91*2.4 ± 0.51
S. aureus3.1 ± 0.21*3.1 ± 0.14*3.1 ± 0.21*3.1 ± 0.23*3.1 ± 0.23*3.2 ± 0.25*2.8 ± 0.25
E. coli2.0 ± 0.112.0 ± 1.082.0 ± 1.102.0 ± 1.152.0 ± 1.151.8 ± 1.441.8 ± 1.11
B. cereus3.3 ± 0.18*3.4 ± 0.23*3.2 ± 0.25*3.1 ± 0.06*3.1 ± 0.093.4 ± 0.50*2.9 ± 0.41
0.2%LAB monoculture2.5 ± 0.34*2.0 ± 0.53*2.2 ± 0.46*1.8 ± 0.27*2.2 ± 0.38*2.2 ± 0.62*1.2 ± 0.25
P. aeruginosa0.8 ± 0.791.0 ± 0.76*0.2 ± 0.380.1 ± 0.881.0 ± 0.86*1.0 ± 0.88*0.7 ± 0.43
S. aureus1.7 ± 0.64*1.6 ± 0.47*1.7 ± 0.52*1.7 ± 0.66*1.7 ± 0.64*1.6 ± 0.54*0.6 ± 0.30
E. coli0.5 ± 0.680.1 ± 0.500.4 ± 0.370.5 ± 0.64*0.6 ± 0.80*0.3 ± 0.560.2 ± 0.62
B. cereus0000000
ΔTTA2%LAB monoculture2.2 ± 0.63*1.5 ± 0.43*1.7 ± 0.65*2.3 ± 0.87*1.8 ± 0.57*1.3 ± 0.23*1.1 ± 0.01
P. aeruginosa0.3 ± 0.120.3 ± 0.100.3 ± 0.090.3 ± 0.050.3 ± 0.070.6 ± 0.03*0.3 ± 0.04
S. aureus1.1 ± 0.21*1.3 ± 0.18*1.5 ± 0.65*1.1 ± 0.21*1.1 ± 0.21*1.3 ± 0.34*0.5 ± 0.02
E. coli0.7 ± 0.200.5 ± 0.150.5 ± 0.080.6 ± 0.090.6 ± 0.060.6 ± 0.060.6 ± 0.01
B. cereus1.3 ± 0.25*1.3 ± 0.31*1.5 ± 0.74*1.3 ± 0.21*1.4 ± 0.21*1.4 ± 0.76*0.7 ± 0.03
0.2%LAB monoculture0.31 ± 0.110.30 ± 0.100.31 ± 0.080.30 ± 0.050.27 ± 0.030.25 ± 0.050.31 ± 0.06
P. aeruginosa0.03 ± 0.010.03 ± 0.010.05 ± 0.020.03 ± 0.010.02 ± 0.010.03 ± 0.020.01 ± 0.02
S. aureus0.05 ± 0.010.01 ± 0.010.05 ± 0.010.02 ± 0.020.05 ± 0.030.05 ± 0.020.02 ± 0.01
E. coli0.08 ± 0.010.50 ± 0.010.52 ± 0.010.5 ± 0.020.07 ± 0.020.23 ± 0.020.28 ± 0.09
B. cereus0.02 ± 0.010.05 ± 0.010.05 ± 0.010.03 ± 0.010.05 ± 0.020.05 ± 0.010.01 ± 0.02
  1. Suspensions of LAB (107 CFU/mL) in MRS broth without sorbic acid were added into the wells with 48-h old biofilms pre-formed by pathogenic bacteria and incubation was continued for the next 24 h. ΔpH and ΔTTA were calculated as a difference between initial and final values. The Total Titratable Acidity (TTA) is expressed as mL of 0.1 M NaOH as required to achieve the final pH of 8.2
  2. * denotes statistically significant difference with probiotic strain L. plantarum 8PA3 (p < 0.05)