|
Ingredients
|
Amounts (g)
|
Preparation procedures
|
|---|
|
1
|
Yeast extract
|
2.5
|
Ingredients 1–4 were put in an Erlenmeyer containing 250 mL of distilled water and the solution was mixed with a magnetic stirrer. Then distilled water was added to total volume of 500 mL and transferred to 1 L flask. The liquid was autoclaved for 20 min at 115 °C and let to cool at ~ 55 °C before pipetting 25 mL onto each petri dish plate.
|
|
2
|
Tryptone
|
5
|
|
3
|
NaCl
|
5
|
|
4
|
Agar powder
|
7.5
|
|
5
|
Water
|
500
|
- Note: The preparation of LB broth follows the same procedures but without agar powder
