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Table 1 Ingredients and preparation of the standard Luria Bertani (LB) agar media

From: Symbiotic bacteria motivate the foraging decision and promote fecundity and survival of Bactrocera dorsalis (Diptera: Tephritidae)

Ingredients Amounts (g) Preparation procedures
1 Yeast extract 2.5 Ingredients 1–4 were put in an Erlenmeyer containing 250 mL of distilled water and the solution was mixed with a magnetic stirrer. Then distilled water was added to total volume of 500 mL and transferred to 1 L flask. The liquid was autoclaved for 20 min at 115 °C and let to cool at ~ 55 °C before pipetting 25 mL onto each petri dish plate.
2 Tryptone 5
3 NaCl 5
4 Agar powder 7.5
5 Water 500
  1. Note: The preparation of LB broth follows the same procedures but without agar powder