Fig. 2
Metallothioneins contribute to antioxidant-mediated growth rescue in the presence of FLC. a Growth spot assay of wild type (H99), and the cmt1Δ, cmt2Δ, cmt1/2Δ mutants on YPD, or YPD supplemented with FLC (32 μg/mL) or FLC and an antioxidant (10 mM PDTC, 1 mM RA, 10 mM AA, 10 mM GSH). b 2 × 106 of wild type cells (H99) or metallothionein mutants were spread on YPD semi-solid media or YPD media supplemented with antioxidants at concentrations as described in a. Discs containing increasing amounts of FLC (25, 50, or 100 μg) were placed on media and the growth inhibition zones were examined after 2 days of incubation at 25 °C. c Graph based on quantification of the results of the experiment described in b (based on three replicates). Error bars indicate standard deviation. Stars indicate significant growth rescue by the antioxidant as compared to the control treatment (p < 0.05)