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Table 1 Richness and diversity estimation of the 16S rDNA, 18S rDNA, and ITS sequencing libraries

From: High-throughput sequencing revealed differences of microbial community structure and diversity between healthy and diseased Caulerpa lentillifera

Sample

OTUs

ACE

Chao1

Shannon

Simpson

Coverage(%)

Effective Tags

16S rDNA

 CK

1217 ± 254 a

1394 ± 219 a

1405 ± 212 a

6.921 ± 0.072 b

0.971 ± 0.004 a

99.730 ± 0.027 a

84,020 ± 5587 a

 SA

1239 ± 56 a

1437 ± 89 a

1465 ± 92 a

7.570 ± 0.402 a

0.982 ± 0.010 a

99.614 ± 0.048 b

87,206 ± 2441 a

 DA

1206 ± 198 a

1422 ± 198 a

1438 ± 196 a

6.909 ± 0.406 b

0.971 ± 0.011 a

99.645 ± 0.019 b

82,879 ± 8562 a

 AO

1022 ± 72 a

1178 ± 79 a

1185 ± 76 a

6.614 ± 0.117 b

0.959 ± 0.002 b

99.734 ± 0.004 a

86,932 ± 5643 a

18S rDNA

 CK

214 ± 25 a

252 ± 43 b

244 ± 30 a

1.288 ± 0.258 c

0.264 ± 0.062 c

99.953 ± 0.009 ab

11,664 ± 3851 c

 SA

209 ± 26 a

262 ± 13 ab

262 ± 20 a

2.729 ± 0.107 a

0.706 ± 0.073 a

99.940 ± 0.002 bc

11,934 ± 1426 c

 DA

240 ± 7 a

291 ± 7 a

292 ± 4 a

3.182 ± 0.497 a

0.763 ± 0.053 a

99.934 ± 0.005 c

14,693 ± 8656 a

 AO

163 ± 5 b

195 ± 22 c

195 ± 14 b

1.922 ± 0.066 b

0.478 ± 0.017 b

99.961 ± 0.009 a

13,682 ± 2948 b

ITS

 CK

118 ± 10 a

129 ± 1 a

133 ± 11 a

3.006 ± 0.110 a

0.785 ± 0.026 a

99.977 ± 0.002 a

77,855 ± 1035 c

 SA

76 ± 5 b

88 ± 9 b

89 ± 8 b

2.423 ± 0.148 b

0.713 ± 0.028 a

99.984 ± 0.003 a

10,744 ± 2269 ab

 DA

59 ± 6 c

78 ± 25 b

78 ± 1 b

1.219 ± 0.615 c

0.354 ± 0.211b

99.983 ± 0.006 a

94,962 ± 9216 b

 AO

60 ± 5 c

69 ± 8 b

70 ± 9 b

1.692 ± 0.181 c

0.495 ± 0.040 b

99.986 ± 0.005 a

11,038 ± 6244 a

  1. The cutoff value was 0.03 (sequence identity 0.97). ACE and Chao1 indices were used to evaluate the community richness, while Shannon and Simpson indices were used to assess the community diversity. The values of mean ± SD of three samples are shown in the table. The different letters superscript indicate significant differences. CK, SA, DA represent the healthy, diseased, and decayed algae samples respectively. AO represents the sediment samples collected from the algae farm