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Table 1 Richness and diversity estimation of the 16S rDNA, 18S rDNA, and ITS sequencing libraries

From: High-throughput sequencing revealed differences of microbial community structure and diversity between healthy and diseased Caulerpa lentillifera

Sample OTUs ACE Chao1 Shannon Simpson Coverage(%) Effective Tags
16S rDNA
 CK 1217 ± 254 a 1394 ± 219 a 1405 ± 212 a 6.921 ± 0.072 b 0.971 ± 0.004 a 99.730 ± 0.027 a 84,020 ± 5587 a
 SA 1239 ± 56 a 1437 ± 89 a 1465 ± 92 a 7.570 ± 0.402 a 0.982 ± 0.010 a 99.614 ± 0.048 b 87,206 ± 2441 a
 DA 1206 ± 198 a 1422 ± 198 a 1438 ± 196 a 6.909 ± 0.406 b 0.971 ± 0.011 a 99.645 ± 0.019 b 82,879 ± 8562 a
 AO 1022 ± 72 a 1178 ± 79 a 1185 ± 76 a 6.614 ± 0.117 b 0.959 ± 0.002 b 99.734 ± 0.004 a 86,932 ± 5643 a
18S rDNA
 CK 214 ± 25 a 252 ± 43 b 244 ± 30 a 1.288 ± 0.258 c 0.264 ± 0.062 c 99.953 ± 0.009 ab 11,664 ± 3851 c
 SA 209 ± 26 a 262 ± 13 ab 262 ± 20 a 2.729 ± 0.107 a 0.706 ± 0.073 a 99.940 ± 0.002 bc 11,934 ± 1426 c
 DA 240 ± 7 a 291 ± 7 a 292 ± 4 a 3.182 ± 0.497 a 0.763 ± 0.053 a 99.934 ± 0.005 c 14,693 ± 8656 a
 AO 163 ± 5 b 195 ± 22 c 195 ± 14 b 1.922 ± 0.066 b 0.478 ± 0.017 b 99.961 ± 0.009 a 13,682 ± 2948 b
ITS
 CK 118 ± 10 a 129 ± 1 a 133 ± 11 a 3.006 ± 0.110 a 0.785 ± 0.026 a 99.977 ± 0.002 a 77,855 ± 1035 c
 SA 76 ± 5 b 88 ± 9 b 89 ± 8 b 2.423 ± 0.148 b 0.713 ± 0.028 a 99.984 ± 0.003 a 10,744 ± 2269 ab
 DA 59 ± 6 c 78 ± 25 b 78 ± 1 b 1.219 ± 0.615 c 0.354 ± 0.211b 99.983 ± 0.006 a 94,962 ± 9216 b
 AO 60 ± 5 c 69 ± 8 b 70 ± 9 b 1.692 ± 0.181 c 0.495 ± 0.040 b 99.986 ± 0.005 a 11,038 ± 6244 a
  1. The cutoff value was 0.03 (sequence identity 0.97). ACE and Chao1 indices were used to evaluate the community richness, while Shannon and Simpson indices were used to assess the community diversity. The values of mean ± SD of three samples are shown in the table. The different letters superscript indicate significant differences. CK, SA, DA represent the healthy, diseased, and decayed algae samples respectively. AO represents the sediment samples collected from the algae farm