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Table 1 Genes that encode proteins involved in the pathogenesis mechanism and/or immune response; these were used as a molecular target for the PCR assay. The function, classification, nucleotide position and sequences of the forward and reverse primers, annealing temperature and amplicon size (in base pairs) of each target gene are described

From: Genetic variation of Mycoplasma hyopneumoniae from Brazilian field samples

Gene

Function

Classification

Nucleotide position

Primer sequences

Annealing temperature (°C)

Amplicon (bp)

mhp 0107

P102-like protein

Surface proteins

67

5′-AGTTTATCAGCCGCTGTTGG-3’

58.5

238

305

5′-GCCCGAACTAAGTCATACCG-3’

mhp 0272

P97-like protein

1627

5′-GTTCGGGCTTGATCTGGTAA-3’

56.6

271

1898

5′-TGCGAAGCAGTCCGATTT-3’

P97

Surface adhesin

2437

5′-CCAGCAGCTAAACCAGTAGC-3’

55

761

3198

5′-AGGATCACCGGATTTTGAATC −3’

mhp0199

Protein P102

1581

5′-TAGCCCCACACAACCGAAAA-3’

58

567

2148

5′-ACCTTGATCAGTTTGAATTGCATCT-3’

mhp 0099

Lipoprotein P95

1297

5′-CCGCTTGATAGCGAGAAGAAA-3’

60

402

1699

5′-GACGGCTTTGACTACCATCTT-3’

mhp 0580

Membrane nuclease lipoprotein

311

5′-TTGGCTTAGCGGAGATAACG-3’

60

237

548

5′-AGCGGTCTGGCTCATTCTAA-3’

mhp 461

Multifunctional leucine aminopeptidase on the surface

299

5′-TCTTCTCGCTAAGAGCCGAT-3’

60

400

699

5′-ACCACCTGAATCAAAAGTAATTCCT-3’

mhp 0360

P37-like ABC transporter substrate-binding lipoprotein

540

5′-TTTACGCCAATCAGCTAGGG-3’

60

210

750

5′-GGCTTTCTTGATCTCCTCTCG-3’

P46

Membrane surface protein

325

5′-AGTCCAGCGCCAAAAGGATT-3’

57

503

828

5′-ACCACCTGCTGGATCTTTGT-3’

mhp 0067

Chaperone protein dnaK

Non-surface proteins

27

5′-CGACCTTGGAACAACAAACTC-3’

60

538

565

5′-CGAAGGTTCCACCGGATAAT-3’

nrdF

Ribonucleotide reductase R2 subunit

691

5′-AGCCCGGAAAAACAAGACGA-3’

60

304

995

5′-GTTTCTTCAGCAAGCGCCAT-3’

mhp372

Lipoprotein

1562

5′-TGCAAAAAGTTGGTGCAGTT-3’

58

579

2141

5′-AGACTTATTCCACCTTCGGCT-3’

mhp 0443

Hypothetical protein

Hypothetical proteins

1667

5′-GAGCATCAGGTTCTGGGGTA-3’

60

222

1889

5′-GAAGGTTGATCCTCGCTCTG-3’

mhp 0660

Lipoprotein

345

5′-GCAGCCCGAAATAACTAGTCC-3’

58.5

283

628

5′-TTCGTGCGTTAGCAACCTG-3’

mhp0418

Lipoprotein

369

5′-TGCTAGTTTTATTCCAAGCCCT-3’

55.8

599

968

5′-CTTTGCACGCGCTGGATTAG-3’

mhp 0487

Putative Mg2+ transporter

775

5′-GCAGGAATTTCACCTCAGGA-3’

56.6

283

1058

5′-AGCGAAATTGCCCTGACA-3’