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Table 1 Genes that encode proteins involved in the pathogenesis mechanism and/or immune response; these were used as a molecular target for the PCR assay. The function, classification, nucleotide position and sequences of the forward and reverse primers, annealing temperature and amplicon size (in base pairs) of each target gene are described

From: Genetic variation of Mycoplasma hyopneumoniae from Brazilian field samples

GeneFunctionClassificationNucleotide positionPrimer sequencesAnnealing temperature (°C)Amplicon (bp)
mhp 0107P102-like proteinSurface proteins675′-AGTTTATCAGCCGCTGTTGG-3’58.5238
3055′-GCCCGAACTAAGTCATACCG-3’
mhp 0272P97-like protein16275′-GTTCGGGCTTGATCTGGTAA-3’56.6271
18985′-TGCGAAGCAGTCCGATTT-3’
P97Surface adhesin24375′-CCAGCAGCTAAACCAGTAGC-3’55761
31985′-AGGATCACCGGATTTTGAATC −3’
mhp0199Protein P10215815′-TAGCCCCACACAACCGAAAA-3’58567
21485′-ACCTTGATCAGTTTGAATTGCATCT-3’
mhp 0099Lipoprotein P9512975′-CCGCTTGATAGCGAGAAGAAA-3’60402
16995′-GACGGCTTTGACTACCATCTT-3’
mhp 0580Membrane nuclease lipoprotein3115′-TTGGCTTAGCGGAGATAACG-3’60237
5485′-AGCGGTCTGGCTCATTCTAA-3’
mhp 461Multifunctional leucine aminopeptidase on the surface2995′-TCTTCTCGCTAAGAGCCGAT-3’60400
6995′-ACCACCTGAATCAAAAGTAATTCCT-3’
mhp 0360P37-like ABC transporter substrate-binding lipoprotein5405′-TTTACGCCAATCAGCTAGGG-3’60210
7505′-GGCTTTCTTGATCTCCTCTCG-3’
P46Membrane surface protein3255′-AGTCCAGCGCCAAAAGGATT-3’57503
8285′-ACCACCTGCTGGATCTTTGT-3’
mhp 0067Chaperone protein dnaKNon-surface proteins275′-CGACCTTGGAACAACAAACTC-3’60538
5655′-CGAAGGTTCCACCGGATAAT-3’
nrdFRibonucleotide reductase R2 subunit6915′-AGCCCGGAAAAACAAGACGA-3’60304
9955′-GTTTCTTCAGCAAGCGCCAT-3’
mhp372Lipoprotein15625′-TGCAAAAAGTTGGTGCAGTT-3’58579
21415′-AGACTTATTCCACCTTCGGCT-3’
mhp 0443Hypothetical proteinHypothetical proteins16675′-GAGCATCAGGTTCTGGGGTA-3’60222
18895′-GAAGGTTGATCCTCGCTCTG-3’
mhp 0660Lipoprotein3455′-GCAGCCCGAAATAACTAGTCC-3’58.5283
6285′-TTCGTGCGTTAGCAACCTG-3’
mhp0418Lipoprotein3695′-TGCTAGTTTTATTCCAAGCCCT-3’55.8599
9685′-CTTTGCACGCGCTGGATTAG-3’
mhp 0487Putative Mg2+ transporter7755′-GCAGGAATTTCACCTCAGGA-3’56.6283
10585′-AGCGAAATTGCCCTGACA-3’