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Fig. 6 | BMC Microbiology

Fig. 6

From: Characterization of LrgAB as a stationary phase-specific pyruvate uptake system in Streptococcus mutans

Fig. 6

The effect of 3-fluoropyruvate (3FP; pyruvate analogue) on lrg promoter (PlrgA) activity and pyruvate uptake. A-D: Measurment of lrg promoter (PlrgA) activity. The PlrgA-gfp reporter strain was grown in a low-glucose (11 mM) FMC medium supplemented by 0 (a), 0.01 (b), 0.1 (c), or 1 mM (d) 3FP. Relative gfp expression (green square) and OD600 (grey circle; OD) were monitored on a plate reader (see Materials and Methods for details). The results are representative of two independent experiments. E-F: Measurement of extracellular pyruvate. The S. mutans UA159 wild type strain was grown in a low-glucose (11 mM) FMC medium supplemented by 0.01 (e) and 1 mM (f) 3FP. For time course measurements of extracellular pyruvate and growth, samples were taken at 1 or 2 h intervals (see Materials and Methods for details). The concentration of pyruvate was determined using an EnzyChrom™ pyruvate assay kit, and growth was measured by the optical density at 600 nm (OD600). Bars indicate the concentration of extracellular pyruvate; solid line with circles indicates growth curve. The results are representative of two independent experiments

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