Fig. 4
Change of lrg promoter (PlrgA) activity during growth in FMC medium supplemented by different concentrations of extracellular pyruvate. The PlrgA-gfp reporter strain was grown in a low-glucose (11 mM) FMC medium supplemented by 0 (a), 1 (b), 2 (c), 5 (d), 10 (e), 20 (f), 40 (g), and 80 mM (h) pyruvate (pyr). Relative gfp expression (green squares) and OD600 (grey circles; OD) were monitored during growth on a plate reader (see Materials and Methods for details). The results are representative of two independent experiments