Fig. 16

Growth curve of Liberibacter crescens inoculum source for temperature, buffer requirements and pH modulation experiments. 15 μL of glycerol stocks of L. crescens were activated in 2 mL of Hi-GI medium, subcultured in 5 mL of Hi-GI, and transferred to a 25 mL of Hi-GI in a 1:20 ratio inoculum: medium. Initial pH of Hi-GI was set to 5.92. Cultures were grown at 250 rpm and 28 °C. The inoculum was collected during middle to late exponential phase of growth at OD₆₀₀~0.6–0.7 after 72 to 96 h prior to washing and inoculation in the different media