Amikacin resistance due to the aphA6 gene in multi-antibiotic resistant Acinetobacter baumannii isolates belonging to global clone 1 from Iran

Table 2 The primers used in this study

Target	Primer	Sequence (5′-3′)	Annealing temperature (°C)	Amplicon length (bp)	Reference
aphA6	aphA6F	CATTTGCGGGGTTTTTAATG	59	837	This study
aphA6	aphA6R	TTAGATAATGCTTGGAATCA	59	837	This study
ISAba125-aphA6	RH573	AAGAAGGCTTTTCAGCCAGA	58	1427	[8]
ISAba125-aphA6	aphA6R	GGACAATCAATAATAGCAAT	58	1427	[22]
aphA6-ISAba125	aphA6F	ATACAGAGACCACCATACAGT	58	1745	[22]
aphA6-ISAba125	RH574	CAAACATGAGGTGCGACAGT	58	1745	[8]
repAci6	gr6FW	AGCAAGTACGTGGGACTAAT	59	662	[12]
repAci6	gr6RV	AAGCAATGAAACAGGCTAAT	59	662	[12]
TnaphA6_L	RH1501	CTTGAGGAAGGGATGGTTGA	59	1930	[9]
TnaphA6_L	aphA6R	GGACAATCAATAATAGCAAT	59	1930	[22]
TnaphA6_R	aphA6F	ATACAGAGACCACCATACAGT	59	2540	[22]
TnaphA6_R	RH1502	TTGCTTTAATCGGTGGTTCC	59	2540	[9]
Repeated sequence 1	RH1398	TTTGACGTTGCTCTTGTTGC	58	991	[9]
Repeated sequence 1	RH1399	TTCTCCCAAGTGGTCAGGTC	58	991	[9]
Repeated sequence 2	RH1394	TGGTTGGCAGAACAAGATGA	58	1372	[9]
Repeated sequence 2	RH1395	TCAAACGATGCAATGGAAGA	58	1372	[9]
Repeated sequence 3	RH1503	GAAGATCCAGAAGCGGGATA	58	1575	[9]
Repeated sequence 3	RH1397	CCATGTTCTTTTCCACATGC	58	1575	[9]

ISSN: 1471-2180