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Table 1 Primers’ sequence used in multiplex PCR for amplification of stx1, stx2 and eaeA genes

From: Escherichia coli O157:H7: distribution, molecular characterization, antimicrobial resistance patterns and source of contamination of sheep and goat carcasses at an export abattoir, Mojdo, Ethiopia

Target gene

Primers sequence (5′-3′) (Forward/reverse)

Amplicon size (bp)

Reference

stx1

ATAAATCGCCATTCGTTGACTAC/ AGAACGCCCACTGAGATCATC

180

[27]

stx2

GGCACTGTCTGAAACTGCTCC/ TCGCCAGTTATCTGACATTCTG

255

[27]

eaeA

GACCCGGCACAAGCATAAGC/ CCACCTGCAGCAACAAGAGG

384

[29]

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