Fig. 5

Response of the hilA promoter to yeast extract is due to transcriptional crosstalk with the flagellar system. Expression from hilA promoter was determined using single-copy transcriptional fusions to GFP as determined using flow cytometry. a. hilA promoter activity in the wild type during growth in TB medium at various concentrations of yeast extract (YE). b. hilA promoter activity in the wild type during growth in TB medium containing 10 mM acetate at various concentrations of yeast extract (YE). c. hilA promoter activity in a ΔfliZ mutant during growth in TB medium at various concentrations of yeast extract (YE). d. hilA promoter activity in a ΔfliZ mutant during growth in TB medium containing 10 mM acetate at various concentrations of yeast extract (YE). Negative control (NC) is the measured fluorescence of wild-type cells not containing the gfp gene during growth in TB medium. Panels A and B are shown for comparative purposes and are the same results as shown in Fig. 2. Analysis of data is provided in Additional file 1: Fig. S10