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Fig. 7 | BMC Microbiology

Fig. 7

From: Quantitative methods for assessing local and bodywide contributions to Wolbachia titer in maternal germline cells of Drosophila

Fig. 7

wsp abundance as indicated by real-time qPCR. Whole fly extracts and ovarian extracts are compared in each experiment. Panels show data from 2 independent plate replicates. ā€œnā€ represents 6 technical replicates from 3 sample tubes. Data from each sample/well represent material from 5 female flies. a and b Absolute counts of wsp gene. Wsp abundance was compared in A) control DMSO vs. rifampicin treatment conditions, and B) un-enriched vs. yeast-enriched treatment conditions. c and d Relative counts, showing a ratio of wsp/rpl32 abundance in c control DMSO vs. rifampicin treatment conditions, and d un-enriched vs. yeast-enriched treatment conditions. Statistical tests were applied as appropriate to each dataset, outlined in Additional file 2: Table S12 and S14

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