Fig. 6

Absolute wsp abundance as indicated by real-time qPCR. Data from each sample/well represent 5 female flies. a Validation that bodywide wsp amplification by qPCR corresponds to Wolbachia infection. “n” represents 6 technical replicates from each of 3 sample tubes. b Test for bodywide wsp abundance changes within assayed timespan. Carrier DMSO and rifampicin conditions are shown. Data from 3 plate replicates are shown in pairs. n = 3 technical replicates from each of 12 wells. c Comparisons of randomly sub-sampled data from DMSO and rifampicin conditions, with alpha set at 0.01 n = 3–12 technical replicates (of total 36). Tests used per replicate: Plate 2 (grey): Welch’s T-test. Plate 3 (black): Mann-Whitney U. d Comparisons of bodywide wsp abundance in un-enriched versus yeast-enriched conditions. n = 3 technical replicates from 12 wells. e Comparing randomly sub-sampled data from un-enriched and yeast-enriched conditions, with alpha set at 0.01 n = 3–12 technical replicates (of total 36). Tests used per replicate: Plate 1 (white): Mann-Whitney U. Plate 2 (grey): Mann-Whitney U. Plate 3 (black): Welch’s T-test