Fig. 1

Approach used for Wolbachia titer analysis in D. melanogaster oogenesis. The workflow is presented for fly preparation, tissue processing, ovariole selection, and image analysis. Morphology and position of the oocyte were among the criteria used for staging individual egg chambers. At the distal tip of the ovariole: blue represents the germline stem cell. At stage 4 and stage 10: half blue, half white: ovals represent individual egg chambers. The blue section represents the oocyte. Shown as purple dots: germline Wolbachia