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Fig. 1 | BMC Microbiology

Fig. 1

From: Peptidoglycan-type analysis of the N-acetylmuramic acid auxotrophic oral pathogen Tannerella forsythia and reclassification of the peptidoglycan-type of Porphyromonas gingivalis

Fig. 1

Autoradiogram of a TLC showing radioactive phosphorylation of digested T. forsythia cell walls with γ-32P-ATP by GlcNAc/MurNAc kinase MurK, yielding GlcNAc-6-phosphate (GlcNAc-632P) and MurNAc 6-phosphate (MurNAc-632P), and by anhMurNAc kinase AnmK of E. coli, yielding MurNAc-632P. Reaction time points were 0 and 1 h. The representative section of the autoradiogram is shown. The running direction of the solvent is indicated by an upward arrow on the left side of the plate

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