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Table 5 The comparison of detection results of different methods in contaminated sleeve-fish

From: Development and evaluation of a rapid and sensitive RPA assay for specific detection of Vibrio parahaemolyticus in seafood

Spiked cells (CFU/25 g sleeve-fish)

Enrichment time (h)

Real-time RPA (min)

Real-time PCR (Ct)

RPA (min)

GB4789.7 (Day)

Viable cell counts (CFU/g)

7

6

8.15

27.05

20

3

8.9 × 102

8

7.35

21.33

20

3

9.1 × 103

10

6

6.33

25.31

20

3

7.5 × 102

8

6.03

20.45

20

3

9.5 × 103

56

6

6.05

17.40

20

3

1.0 × 105

8

5.72

18.53

20

3

3.1 × 105

  1. ND Not detected