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Table 5 The comparison of detection results of different methods in contaminated sleeve-fish

From: Development and evaluation of a rapid and sensitive RPA assay for specific detection of Vibrio parahaemolyticus in seafood

Spiked cells (CFU/25 g sleeve-fish) Enrichment time (h) Real-time RPA (min) Real-time PCR (Ct) RPA (min) GB4789.7 (Day) Viable cell counts (CFU/g)
7 6 8.15 27.05 20 3 8.9 × 102
8 7.35 21.33 20 3 9.1 × 103
10 6 6.33 25.31 20 3 7.5 × 102
8 6.03 20.45 20 3 9.5 × 103
56 6 6.05 17.40 20 3 1.0 × 105
8 5.72 18.53 20 3 3.1 × 105
  1. ND Not detected