Table 4 The comparison of detection results of different methods in contaminated codfish
Spiked cells (CFU/25 g codfish) | Enrichment time (h) | Real-time RPA (min) | Real-time PCR (Ct) | RPA (min) | GB4789.7 (Day) | Viable cell counts (CFU/g) |
|---|---|---|---|---|---|---|
1 | 6 | 12.02 | 30.64 | 20 | ND | 0 |
8 | 11.03 | 29.18 | 20 | ND | 0 | |
10 | 6 | 6.07 | 25.12 | 20 | 3 | 5.2 × 103 |
8 | 5.37 | 20.43 | 20 | 3 | 7.1 × 103 | |
42 | 6 | 5.07 | 17.45 | 20 | 3 | 2.2 × 104 |
8 | 4.20 | 17.15 | 20 | 3 | 7.2 × 104 |