Table 3 The comparison of detection results of different methods in contaminated oyster sauce
Spiked cells (CFU/25 g oyster sauce) | Enrichment time (h) | Real-time RPA (min) | Real-time PCR (Ct) | RPA (min) | GB4789.7 (Day) | Viable cell counts (CFU/g) |
|---|---|---|---|---|---|---|
4 | 6 | 5.02 | 32.48 | 20 | 3 | 4.4 × 102 |
8 | 3.35 | 21.45 | 20 | 3 | 4.8 × 102 | |
25 | 6 | 4.03 | 27.15 | 20 | 3 | 6.3 × 103 |
8 | 2.88 | 20.36 | 20 | 3 | 4.1 × 103 | |
80 | 6 | 3.72 | 21.34 | 20 | 3 | 1.0 × 104 |
8 | 2.72 | 16.32 | 20 | 3 | 1.0 × 104 |