Table 2 Primer and probe sequences for V. parahaemolyticus real-time PCR, RPA and real-time RPA assay
Method | Name | Sequence 5′-3’Amplication | size (bp) |
|---|---|---|---|
Real-time RPA | RPA-FP | CGAAGAAAGCGAAAACGGCAACGTCAGGCGA | 168 |
RPA-RP | CAGATAATTTCTCACCCATCGCCGATTCAACC | ||
exo Probe | GGTTTGACAGCCGTTGTTTCAGTAAAAGTGCC [FAM-dT]-THF-[BHQ-dT]TCCAAAATTCTCGAGCC | ||
Real-time PCR | PCR-FP | CGGTAGTAAACGCACTGTCAGAA | 77 |
PCR-RP | ACGGTAAGTTTGCGTGTGGAT | ||
PCR-Probe | FAM- TGGTACTAACCATCCATCGTGGCGGTC -BHQ1 |