Fig. 3
Performance of real-time RPA for detection of V. parahaemolyticus. a. Fluorescence development over time using a dilution range of 106 to 100 copies of the standard DNA as described above. b. Semi-logarithmic regression of the data collected from eight runs on the standard DNA using GraphPad Prism 7.0 (GraphPad Software Inc., San Diego, CA). The runtimes of real-time RPA were approximately 4.5 to 13 min for 106 to 102 copies. c. A probit regression analysis. The limit of detection of the real-time RPA was approximately 1.02 × 102 copies/reaction in 95% of cases and indicated by a rhomboid