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Fig. 3 | BMC Microbiology

Fig. 3

From: Development and evaluation of a rapid and sensitive RPA assay for specific detection of Vibrio parahaemolyticus in seafood

Fig. 3

Performance of real-time RPA for detection of V. parahaemolyticus. a. Fluorescence development over time using a dilution range of 106 to 100 copies of the standard DNA as described above. b. Semi-logarithmic regression of the data collected from eight runs on the standard DNA using GraphPad Prism 7.0 (GraphPad Software Inc., San Diego, CA). The runtimes of real-time RPA were approximately 4.5 to 13 min for 106 to 102 copies. c. A probit regression analysis. The limit of detection of the real-time RPA was approximately 1.02 × 102 copies/reaction in 95% of cases and indicated by a rhomboid

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