Table 3 RT-qPCR primer sequences (5′ to 3′)
Gene | Primer sequence (5′ → 3′) |
|---|---|
TNF-α | Forward: CATCTTCTCAAAATTCGAGTGACAA |
Reverse: TGGGAGTAGACAAGGTACAACCC | |
IL-1β | Forward: TCACAGCAGCACATCAACAA |
Reverse: TGTCCTCATCCTGGAAGGTC | |
IL-6 | Forward: ACTCACCTCTTCAGAACGAATTG |
Reverse: CCATCTTTGGAAGGTTCAGGTTG | |
Claudin-1 | Forward: TCTACGAGGGACTGTGGATG |
Reverse: TCAGATTCAGCTAGGAGTCG | |
E-Cadherin | Forward: CCACCAAAGTCACGCTGAATAC |
Reverse: GGAGTTGGGAAATGTGAGCAA | |
Occludin | Forward: ACTGGGTCAGGGAATATCCA |
Reverse: TCAGCAGCAGCCATGTACTC | |
ZO-1 | Forward: AGGCTACCTTTGTATTCTC |
Reverse: TAGGGCACAGTATTGTATC | |
β-Actin | Forward: CTCCTACCACACCCATTCTCATCC |
Reverse: GCAATGCCTGGGTACATGGTGG |