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Table 1 Results of ChAP-Seq analysis with C. glutamicum ATCC13032ΔftsR/pAN6-ftsR-Strep. The nine DNA regions that showed a ≥ 50-fold higher coverage than the entire genome (coverage 40) in the ChAP-Seq analysis with FtsR-Strep were analyzed with respect to the location of the peak within the genome and the mRNA levels of the genes in this region. Based on that, a prediction was made which genes could be potential FtsR targets. The arrows indicate whether the genes are encoded on the leading (→) or lagging strand (←) within the genome. Graphical representations of selected peaks as derived from the ChAP-Seq analysis are shown in Additional file 1: Figure S10

From: The conserved actinobacterial transcriptional regulator FtsR controls expression of ftsZ and further target genes and influences growth and cell division in Corynebacterium glutamicum

No.a Coveragea Peak position DNA microarray 13032ΔftsR/13032 Potential FtsR target genes
   in gene downstream 3′-end of upstream of Gene mRNA ratio p-value  
1 (1) 28,811 (4781) cg2478 (NCgl2178, penicillin binding protein) ← cg2477 (NCgl2177, conserved hypothetical protein) ← cg2478 0.75 0.11 cg2477 potentially activated by FtsR
cg2477 0.24 0.01
2 (5) 22,757 (934) cg3182 (NCgl2777, cop1, trehalose corynemycolyl transferase) ← cg3182 1.70 0.20 cop1-cg3181-cg3180 potentially repressed by FtsR
cg3181 2.30 0.05
cg3180 1.91 0.05
cg3181 (NCgl2776, put. Secreted protein) ← cg3180 (NCgl2775, put. Secreted protein) ← cg3183 (NCgl2778, put. transposase) → cg3183 n.a. n.a.
3 (2) 4437 (1556) cg2367 (NCgl2076, ftsQ, cell division septal protein) ← cg2366 (NCgl2075, ftsZ, cell division GTPase) ← cg2367 1.20 0.02 ftsZ activated by FtsR
cg2366 0.35 0.02
4 (11) 3899 (373) cg0470 (NCgl0381, htaB, heme transport-associated protein) → cg0469 (NCgl0380, hmuV) → cg0471 (NCgl0382, htaC, heme-transport associated protein) → cg0469 1.41 < 0.01
cg0470 1.45 0.09
cg0471 1.55 0.03
5 (12) 3836 (365) cg0852 (NCgl0712, conserv. Hypoth. protein) ← cg0852 0.62 0.01 cg0852 potentially activated by FtsR
cg0853 (NCgl0713, conserv. Hypoth. protein) → cg0854 (NCgl0714, pmmA, phosphomannomutase) → cg0855 (NCgl0715, conserv. Hypoth. protein) → cg0856 (NCgl0716, mannose-6-phosphate isomerase) → cg0853 1.03 0.29
cg0854 0.89 0.25
cg0855 1.18 0.08
cg0856 1.09 0.03
6 (3) 3630 (1529) cg3392 (oxiE, idhA2, NCgl2958) → (myo-inositol 2-dehydrogenase) cg3393 (NCgl2959, phoC, secreted cell-wall-associated phosphatase) → cg3392 1.25 0.03 phoC potentially repressed by FtsR
cg3393 1.60 0.03
7 (15) 2871 (315) cg2695 (NCgl2368, ATPase of ABC transporter) ← cg2697 (ssb, NCgl2370, single-strand DNA-binding protein) ← cg2694 (NCgl2367, put. Phosphodiesterase, nucleotide pyrophosphatase) ← cg2693 (NCgl2366, put. Phosphodiesterase, nucleotide pyrophosphatase) ← cg2693 1.31 0.03
cg2694 1.29 0.05
cg2695 1.06 0.42
cg2697 1.04 0.24
8 (6) 2355 (858) cg2518 (NCgl2213, put. Secreted protein) → cg2519 (NCgl2214, conserv. Hypoth. protein) ← cg2520 (NCgl2215, hypoth. protein) ← cg2521 (NCgl2216, fadD15, long chain fatty acid CoA ligase) ← cg2518 0.81 0.18
cg2519 0.49 0.03
cg2520 0.92 0.45
cg2521 0.91 0.43
9 (8) 2248 cg0839 (NCgl0701) ← (hypoth. protein) cg0840 (NCgl0702, conserv. Hypoth. protein) ← cg0838 (NCgl0700, ATP-depend. helicase) ← cg0838 4.61 0.03 cg0838 potentially repressed
cg0839 2.45 0.16 by FtsR
cg0840 2.45 0.19
  1. a The numbers in brackets represent the result of a second, independent experiment. Please note that the overall coverage was lower in the second experiment