Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil

Koga, Vanessa L.; Maluta, Renato P.; da Silveira, Wanderley D.; Ribeiro, Renan A.; Hungria, Mariangela; Vespero, Eliana C.; Nakazato, Gerson; Kobayashi, Renata K. T.

doi:10.1186/s12866-019-1550-3

Table 1 Oligonucleotide used for amplification in the PCR

From: Characterization of CMY-2-type beta-lactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil

Target	Gene	Oligonucleotide sequence (5′ to 3′)	References
bla_ESBL	bla_CTX-M-1 group	F- AAA AAT CAC TGC GCC AGT TC	[17]
	bla_CTX-M-1 group	R- AGC TTA TTC ATC GCC ACG TT
	bla_CTX-M2 group	F- CGA CGC TAC CCC TGC TAT T
	bla_CTX-M2 group	R- CCA GCG TCA GAT TTT TCA GG
	bla_CTX-M8 group	F- TCG CGT TAA GCG GAT GAT GC
	bla_CTX-M8 group	R- AAC CCA CGA TGT GGG TAG C
	bla_CTX-M9 group	F- CAA AGA GAG TGC AAC GGA TG
	bla_CTX-M9 group	R- ATT GGA AAG CGT TCA TCA CC
	bla_CTX-M25 group	F- GCA CGA TGA CAT TCG GG
	bla_CTX-M25 group	R- AAC CCA CGA TGT GGG TAG C
	bla_TEM	F- CAT TTC CGT GTC GCC CTT ATT C	[18]
	bla_TEM	R- CGT TCA TCC ATA GTT GCC TGA C	[18]
	bla_SHV	F- CAC TCA AGG ATG TAT TGT G	[19]
	bla_SHV	R- TTA GCG TTG CCA GTG CTC G	[19]
bla_pAmpC	bla_MOX-1, bla_MOX-2, bla_CMY-1, bla_CMY-8 to bla_CMY-11	F- GCT GCT CAA GGA GCA CAG GAT	[7]
		R- CAC ATT GAC ATA GGT GTG GTG C
	bla_LAT-1 to bla_LAT-4, bla_CMY-2 to bla_CMY-7, bla_BIL-1	F- TGG CCA GAA CTG ACA GGC AAA
		R- TTT CTC CTG AAC GTG GCT GGC
	bla_DHA-1 and bla_DHA-2	F- AAC TTT CAC AGG TGT GCT GGG T
	bla_DHA-1 and bla_DHA-2	R- CCG TAC GCA TAC TGG CTT TGC
	bla_ACC	F- AAC AGC CTC AGC AGC CGG TTA
	bla_ACC	R- TTC GCC GCA ATC ATC CCT AGC
	bla_MIR-1T, bla_ACT-1	F- TCG GTA AAG CCG ATG TTG CGG
	bla_MIR-1T, bla_ACT-1	R- CTT CCA CTG CGG CTG CCA GTT
	bla_FOX-1 to bla_FOX-5b	F- AAC ATG GGG TAT CAG GGA GAT G
	bla_FOX-1 to bla_FOX-5b	R- CAA AGC GCG TAA CCG GAT TGG
	bla_CMY-2, bla_CMY-4, bla_CMY-6, bla_CMY-7, bla_CMY-12, bla_CMY-13, bla_CMY-14, bla_CMY-18, bla_LAT-3	F- ATG ATG AAA AAA TCG TTA TGC TGC	[20]
		R- GCT TTT CAA GAA TGC GCC AGG	[20]
PMQR	qnrA	F- AGA GGA TTT CTC ACG CCA GG	[21]
	qnrA	R- TGC CAG GCA CAG ATC TTG AC
	qnrB	F- GGM ATH GAA ATT CGC CAC TG
	qnrB	R- TTT GCY GYY CGC CAG TCG AA
	qnrS	F- GCA AGT TCA TTG AAC AGG GT
	qnrS	R- TCT AAA CCG TCG AGT TCG GCG
Tetracycline resistance	tetA	F- GCC TTT CCT TTG GGT TCT CT	[22]
	tetA	R- TGT CCG ACA AGT TGC ATG AT
	tetB	F- GCT TTC AGG GAT CAC AGG AG
	tetB	R- GAC CAA GAC CCG CTA ATG AA
Sulfonamide resistance	sulI	F- ACG AGA TTG TGC GGT TCT TC	[22]
	sulI	R- GGT TTC CGA GAT GGT GAT TG
	sulII	F- CCG TCT CGC TCG ACA GTT AT
	sulII	R- GTG TGT GCG GAT GAA GTC AG
Insertion sequence	ISEcp1 - CMY	F- AAA AAT GAT TGA AAG GTG GT	[7, 23]
Insertion sequence	ISEcp1 - CMY	R- TTT CTC CTG AAC GTG GCT GGC	[7, 23]

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