Table 2 Primers used in this study
Primer | Oligonucleotide sequence (5′-3′) |
|---|---|
Complementation | |
 MR1-hemH1-F | 5′- GTGAGCTCTATGCGACTGAATGTTAGCC − 3′ |
 MR1-hemH1-R | 5′- GCTCTAGATCATTCTGCACTTATCCAAG − 3′ |
 MR1-HemH2-F | 5′- GGAATTCAGAAATGGGTCACGCTGC − 3′ |
 MR1-HemH2-R | 5′- GCTCTAGAGCTTACAGATACGGCTTCACC − 3′ |
 PV4-hemH1-F | 5′- GGAATTCTAAAGTGAAGCGCTATATGAGC − 3′ |
 PV4-hemH1-R | 5′- GCTCTAGAGCAGTGATTTATGCCGTTTACC − 3’ |
 pET28a-KatB-F | 5′- GGAATTCATGAGTCAACAGTATTTAAC − 3’ |
 pET28a-KatB-R | 5′- GCAAGCTTTTATAACCCCAGCGCCATTTTG − 3’ |
 pET28a-cymA-F | 5′- GGAATTCATGAACTGGCGTGCACTATTT − 3’ |
 pET28a-cymA-R | 5′- GCAAGCTTTTATCCTTTTGGATAGGGGTG − 3’ |
Mutagenesis | |
 MR-hemH1_5O | 5′- CAGAGCTCTCGTCGGCCAAAATAGAATA-3’ |
 MR-hemH1_5I | 5′- GACTGGCTTAGGTCGTCTCTCCAAGGCGTGAAAAGTGTCG-3’ |
 MR-hemH1_3I | 5′- AGAGACGACCTAAGCCAGTCGACGACCCGAGGATCACTTA-3’ |
 MR-hemH1_3O | 5′- GTGAGCTCGATGGCGGCAAACGGTATTA − 3’ |
 MR-hemH2_5O | 5′- CAGAGCTCGATGAACGCTAAAACCGTA − 3’ |
 MR-hemH2_5I | 5′- GACTGGCTTAGGTCGTCTCTCGCTCAATGATAATGATGA − 3’ |
 MR-hemH2_3I | 5′- AGAGACGACCTAAGCCAGTCGGATAGCTTAGCCGTTTGT − 3’ |
 MR-hemH2_3O | 5′- GTGAGCTC ACTGCGAGTAATTGTGGTT − 3’ |
 PV4-hemH1_5O | 5′- AGAGCTCGTACGTTCACCGCACTCTGA − 3’ |
 PV4-hemH1_5I | 5′- TGCATCGAGTTGATTGTCGCATCACTGCCCGATGCTATTT − 3’ |
 PV4-hemH1_3I | 5′- GCGACAATCAACTCGATGCATAGGCTAGCACCCACCCTTG − 3’ |
 PV4-hemH1_3O | 5′- AGAGCTCGTTAAAGCGGGAACGCCTCT − 3’ |
QRT-PCR | |
 QRT-PV4-hemH1-F | 5′- CAGTGTCAACGTACTGCCGA − 3’ |
 QRT-PV4-hemH1-R | 5′- ATAAAGCTCTCCTTGCCGCC − 3’ |
 QRT-PV4-hemH2-F | 5′- ATTTCTGCATGCGTTACGGC − 3’ |
 QRT-PV4-hemH2-R | 5′- GCCAGCGCCGCAATATAATC − 3’ |
 QRT-MR-hemH1-F | 5′- CAACGGATTTAAGCGCCACCT − 3’ |
 QRT-MR-hemH1-R | 5′- CTGACCATGGGTTTTCCAATG − 3’ |
 QRT-MR-hemH2-F | 5′- GGTTTTACCGCTCTATCCGC − 3’ |
 QRT-MR-hemH2-R | 5′- CCGTATAGGGTTGCAACCAC − 3’ |
 QRT-MR-rpoE2-F | 5′- CGGGTACAGCATAACCGAGAAG − 3’ |
 QRT-MR-rpoE2-R | 5′- AATCATGTTGTGTCTCCAAAAAGC − 3’ |
 QRT-MR-cymA-F | 5′- CAAGTACAGATGCGTTCTG − 3’ |
 QRT-MR-cymA-R | 5′- GTAAGCCAATGCTTTGTCG − 3’ |
 QRT-MR-napB-F | 5′- GGATTAGGCTTAACGGCTACGGC − 3’ |
 QRT-MR- napB -R | 5′- TGTGGGTAAAAACATGGCGTG − 3’ |
 QRT-MR-hmuX-F | 5′- CCAGATTTAATGCCTGCACAA − 3’ |
 QRT-MR-hmuX-R | 5′- GCATGCCATCCAATTTAAGAT − 3’ |
 QRT-MR-exbB-F | 5′- GCATTCCTCGCCTTGATGAT − 3’ |
 QRT-MR-exbB-R | 5′- GCTTGCAGCTCCCTTTGTTG − 3’ |
 QRT-MR-hmuC-F | 5′- GATCGTTTATCGTCTGGCGAG − 3’ |
 QRT-MR-hmuC-R | 5′- GCTTCAGCTTCACCCAGTAGC − 3’ |
 QRT-MR-hemA-F | 5′- CACAGTTGCCTTAACCATTG − 3’ |
 QRT-MR-hemA-R | 5′- CTCACACATGCTTTGGGCAC − 3’ |
 QRT-MR-hemB-F | 5′- GCAACGCGCAGTGCGTGAAC − 3’ |
 QRT-MR-hemB-R | 5′- GTAGGCCATGATTTGAGTG − 3’ |
 QRT-MR-hemD-F | 5′- CTGACGATACGTTTAAAGCC − 3’ |
 QRT-MR-hemD-R | 5′- GCTTCACGGCCACCAACAC − 3’ |
 QRT-MR-hemF-F | 5′- CACCCATGCCAACGTGCGC − 3’ |
 QRT-MR-hemF-R | 5′- CTTTTATCGAAGCCTGCTTG − 3’ |
 QRT-MR-hemG-F | 5′- CAGATGTTGATAAGGTATTG − 3’ |
 QRT-MR-hemG-R | 5′- CAGATCATTGTGCGGTCGAA − 3’ |
 QRT-16S-F | 5′- TAATGGCTCACCAAGGCGAC − 3’ |
 QRT-16S-R | 5′- GGAGTTAGCCGGTCCTTCTTC − 3’ |
RT-PCR | |
 MR-hemH1-FTF | 5′- CAACGGATTTAAGCGCCACCT − 3’ |
 MR -hemH1-RTR | 5′- CTGACCATGGGTTTTCCAATG − 3’ |
 MR -hemH2-FTF | 5′- GGTTTTACCGCTCTATCCGC − 3’ |
 MR -hemH2-RTR | 5′- CCGTATAGGGTTGCAACCAC − 3’ |
 MR -rpoE2-FTF | 5′- CGGGTACAGCATAACCGAGAAG − 3’ |
 MR -rpoE2-RTR | 5′- AATCATGTTGTGTCTCCAAAAAGC − 3’ |
 16S rRNA-FT-F | 5′-GTTGGAAACGACTGCTAATACC − 3’ |
 16S rRNA-RT-R | 5′-GGTCCTTCTTCTGTAGGTAACG − 3’ |