Table 1 Bacterial strains and plasmids
Strain | Description | Source or reference |
|---|---|---|
Escherichia coli WM3064 | thrB1004 pro thi rpsL hsdS lacZDM15 RP4–1360 (araBAD)567 dapA1341::[erm pir(wt)] | W. Metcalf |
E. coli TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZDM15 Δ lacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Smr) endA1 nupG | Invitrogen |
E. coli EC100D+ | F− mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG pir+(DHFR) | Epicentre Technologies |
E. coli DH5α | F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK− mK+) phoA λ– | Takara |
Shewanella oneidensis MR-1 | Dissimilatory metal-reducing strain isolated from Lake Oneida, New York | [40] |
MR-1ΔhemH1 | In-frame deletion mutant of hemH1 gene (SO_2019) derived from MR-1 | This study |
MR-1ΔhemH2 | In-frame deletion mutant of hemH2 gene (SO_3348) derived from MR-1 | This study |
MR-1ΔhemH1ΔhemH2 | In-frame deletion double mutant of hemH1 (SO_2019) and hemH2 (SO_3348) derived from MR-1 | This study |
Shewanella loihica PV-4 | Iron-rich microbial mat at a hydrothermal vent of Loihi Seamount, Pacific Ocean | [41] |
Modified Shewanella loihica PV-4 | pstI (Shew_0993) and pstM (Shew_0992) double deletion double mutant derived from PV-4 | This study |
PV-4 ΔhemH1 | In-frame deletion mutant of hemH1 (Shew_2229) derived from the modified PV-4 | This study |
Plasmids | ||
 pminiHmarRB1 | mariner transposon delivery plasmid R6K, Kmr | [3] |
 pDS3.0 | Suicide vector derived from pCDV224; Ampr, Gmr, sacB | [42] |
 pDS3.0-PV-hemH1ko | Suicide plasmid for deletion of hemH1 derived from PV-4 | This study |
 pDS3.0-MR-hemH1ko | Suicide plasmid for deletion of hemH1 derived from MR-1 | This study |
 pDS3.0-MR-hemH2ko | Suicide plasmid for deletion of hemH2 derived from MR-1 | This study |
 pHERD30T | Shuttle vector with pBAD promoter, Gmr | [43] |
 pHERD30T-hemH1slo | pHERD30T containing the hemH1 derived from PV-4, Gmr | This study |
 pHERD30T-hemH1so | pHERD30T containing the hemH1 derived from MR-1, Gmr | This study |
 pHERD30T-hemH2so | pHERD30T containing the hemH2 derived from MR-1, Gmr | This study |
 pET28a | Expression vector with T7lac promoter | Novogene |
 pET28a-katB | Overexpression construct of katB | This study |
 pET28a-cymA | Overexpression construct of cymA | This study |