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Table 1 Bacterial strains and plasmids

From: Differential gene content and gene expression for bacterial evolution and speciation of Shewanella in terms of biosynthesis of heme and heme-requiring proteins

StrainDescriptionSource or reference
Escherichia coli WM3064thrB1004 pro thi rpsL hsdS lacZDM15 RP4–1360 (araBAD)567 dapA1341::[erm pir(wt)]W. Metcalf
E. coli TOP10F mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZDM15 Δ lacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Smr) endA1 nupGInvitrogen
E. coli EC100D+F mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG pir+(DHFR)Epicentre Technologies
E. coli DH5αF endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK mK+) phoA λTakara
Shewanella oneidensis MR-1Dissimilatory metal-reducing strain isolated from Lake Oneida, New York[40]
MR-1ΔhemH1In-frame deletion mutant of hemH1 gene (SO_2019) derived from MR-1This study
MR-1ΔhemH2In-frame deletion mutant of hemH2 gene (SO_3348) derived from MR-1This study
MR-1ΔhemH1ΔhemH2In-frame deletion double mutant of hemH1 (SO_2019) and hemH2 (SO_3348) derived from MR-1This study
Shewanellaloihica PV-4Iron-rich microbial mat at a hydrothermal vent of Loihi Seamount, Pacific Ocean[41]
Modified Shewanellaloihica PV-4pstI (Shew_0993) and pstM (Shew_0992) double deletion double mutant derived from PV-4This study
PV-4 ΔhemH1In-frame deletion mutant of hemH1 (Shew_2229) derived from the modified PV-4This study
Plasmids
 pminiHmarRB1mariner transposon delivery plasmid R6K, Kmr[3]
 pDS3.0Suicide vector derived from pCDV224; Ampr, Gmr, sacB[42]
 pDS3.0-PV-hemH1koSuicide plasmid for deletion of hemH1 derived from PV-4This study
 pDS3.0-MR-hemH1koSuicide plasmid for deletion of hemH1 derived from MR-1This study
 pDS3.0-MR-hemH2koSuicide plasmid for deletion of hemH2 derived from MR-1This study
 pHERD30TShuttle vector with pBAD promoter, Gmr[43]
 pHERD30T-hemH1slopHERD30T containing the hemH1 derived from PV-4, GmrThis study
 pHERD30T-hemH1sopHERD30T containing the hemH1 derived from MR-1, GmrThis study
 pHERD30T-hemH2sopHERD30T containing the hemH2 derived from MR-1, GmrThis study
 pET28aExpression vector with T7lac promoterNovogene
 pET28a-katBOverexpression construct of katBThis study
 pET28a-cymAOverexpression construct of cymAThis study