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Fig. 7 | BMC Microbiology

Fig. 7

From: Differential gene content and gene expression for bacterial evolution and speciation of Shewanella in terms of biosynthesis of heme and heme-requiring proteins

Fig. 7

The uptake of exogenous hemin depends on TonB1 energy transduction system in S. oneidensis MR-1. a Organization of the putative hemin uptake gene cluster in MR-1. The region corresponds to the open reading frames from SO_3667 to SO_3675 (left to right); b Effects of exogenous hemin supplement on the PPIX production of MR-1ΔhemH1ΔhemH2. Ultraviolet-visible spectrograms of the bacterial extract (the hemH1 and hemH2 double mutant). The absorbance was measured at a wavelength 405 nm with a spectrometer (UV-1800, Mapada) and quartz cuvettes; c Effects of exogenous hemin supplement on the transcription of hemA, hemB, hemD, hemF, hemG genes in the double mutant MR-1ΔhemH1ΔhemH2; d Transcriptional analyses of hmuX, exbB and hmuC in the wild-type strain and the hemH1-hemH2 double mutants of MR-1. Effect of exogenous hemin supplement (0.1 μg/ml, 5 μg/ml, 40 μg/ml) on the transcription of hmuX, exbB and hmuC in the hemH1-hemH2 double mutants of MR-1

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