Fig. 2
Effects of supplemented hemin concentrations on the bacterial growth of the hemH1 and hemH2 double mutant. a The hemH1 and hemH2 double mutant exhibited red-colored phenotype with supplement of hemin (10 μg/ml) while the double mutant could not grow without supplement of hemin. In the genetic complementation analyses, the plasmid borne wild-type hemH1 or hemH2 gene restored phenotype of the hemH1 and hemH2 double mutant to that of the wild-type strain carrying the empty vector with or without supplement of hemin; b The bacterial strains were grown in the LB broth containing 0, 0.001, 0.01, 0.1, 1, 10, 100 μg/ml of hemin and 15 μg /ml of gentamycin, and incubated at 28 °C for 18 h; c The growth of the MR-1, MR-1ΔhemH1, MR-1ΔhemH2, MR-1ΔhemH1ΔhemH2 in the LB broth with 0, 0.1, 1 μg/ml of hemin over time was measured. Growth was determined by measuring OD600 values