Table 1 Bacterial strains and plasmids
Strain | Genotype | Constructiona | Source |
|---|---|---|---|
B. anthracis | |||
 Sterne 34F2 | pXO1+ pXO2− |  | P. Hanna |
 DPBa89 | ∆ypeB | pDPV392 > 34F2 | [20] |
 DPBa127 | ∆ypeB::pDPV424 (YpeB1–446-His6 ErR) | pDPV424 > DPBa89 | [20] |
 DPBa204 | ∆ypeB::pDPV476 (YpeBK437C-His6 ErR) | pDPV476 > DPBa89 | This study |
 DPBa205 | ∆ypeB::pDPV477 (YpeBS358C-His6 ErR) | pDPV477 > DPBa89 | This study |
 DPBa210 | ∆ypeB::pDPV478 (YpeBT328C-His6 ErR) | pDPV478 > DPBa89 | This study |
 DPBa211 | ∆ypeB::pDPV479 (YpeBM282C-His6 ErR) | pDPV479 > DPBa89 | This study |
 DPBa212 | ∆ypeB::pDPV480 (YpeBY339C-His6 ErR) | pDPV480 > DPBa89 | This study |
 DPBa213 | ∆ypeB::pDPV481 (YpeBE314C-His6 ErR) | pDPV481 > DPBa89 | This study |
 DPBa214 | ∆ypeB::pDPV482 (YpeBV355C-His6 ErR) | pDPV482 > DPBa89 | This study |
 DPBa215 | ∆ypeB::pDPV483 (YpeBV324C-His6 ErR) | pDPV483 > DPBa89 | This study |
 DPBa216 | ∆ypeB::pDPV484 (YpeBT420C-His6 ErR) | pDPV484 > DPBa89 | This study |
 DPBa217 | ∆ypeB::pDPV485 (YpeBA342C-His6 ErR) | pDPV485 > DPBa89 | This study |
 DPBa218 | ∆ypeB::pDPV486 (YpeBV435C-His6 ErR) | pDPV486 > DPBa89 | This study |
 DPBa219 | ∆ypeB::pDPV487 (YpeBQ438C-His6 ErR) | pDPV487 > DPBa89 | This study |
 DPBa220 | ∆ypeB::pDPV488 (YpeB∆25–203 K437C-His6 ErR) | pDPV488 > DPBa89 | This study |
 DPBa221 | pDPV488 (YpeB∆25–203 K437C-His6 ErR) | pDPV488 > 34F2 | This study |
 DPBa222 | ∆ypeB::pDPV489 (YpeB∆25–203 S358C-His6 ErR) | pDPV489 > DPBa89 | This study |
 DPBa223 | pDPV489 (YpeB∆25–203 S358C-His6 ErR) | pDPV489 > 34F2 | This study |
E.coli | |||
 BTH101 |  |  | Euromedex |
 DPVE545 | pKT25-zip + pUT18C-zip | pKT25-zip + pUT18C-zip > BTH101 | Euromedex |
 DPVE546 | pKT25 + pUT18C |  | This study |
 DPVE547 | p25-YpeB21–446 + p18-YpeB21–446 | pDPV494 + pDPV490 > BTH101 | This study |
 DPVE548 | p25-YpeB21–446 + p18-YpeB21–202 | pDPV494 + pDPV491 > BTH101 | This study |
 DPVE549 | p25-YpeB21–446 + p18-YpeB203–446 | pDPV494 + pDPV492 > BTH101 | This study |
 DPVE550 | p25-YpeB21–446 + p18-SleB125–253 | pDPV494 + pDPV493 > BTH101 | This study |
 DPVE551 | p25-YpeB21–202 + p18-YpeB21–446 | pDPV495 + pDPV490 > BTH101 | This study |
 DPVE552 | p25-YpeB21–202 + p18-YpeB21–202 | pDPV495 + pDPV491 > BTH101 | This study |
 DPVE553 | p25-YpeB21–202 + p18-YpeB203–446 | pDPV495 + pDPV492 > BTH101 | This study |
 DPVE554 | p25-YpeB21–202 + p18-SleB125–253 | pDPV495 + pDPV493 > BTH101 | This study |
 DPVE555 | p25-YpeB203–446 + p18-YpeB21–446 | pDPV496 + pDPV490 > BTH101 | This study |
 DPVE556 | p25-YpeB203–446 + p18-YpeB21–202 | pDPV496 + pDPV491 > BTH101 | This study |
 DPVE557 | p25-YpeB203–446 + p18-YpeB203–446 | pDPV496 + pDPV492 > BTH101 | This study |
 DPVE558 | p25-YpeB203–446 + p18-SleB125–253 | pDPV496 + pDPV493 > BTH101 | This study |
 DPVE559 | p25-SleB125–253 + p18-YpeB21–446 | pDPV497 + pDPV490 > BTH101 | This study |
 DPVE560 | p25-SleB125–253 + p18-YpeB21–202 | pDPV497 + pDPV491 > BTH101 | This study |
 DPVE561 | p25-SleB125–253 + p18-YpeB203–446 | pDPV497 + pDPV492 > BTH101 | This study |
 DPVE562 | p25-SleB125–253 + p18-SleB125–253 | pDPV497 + pDPV493 > BTH101 | This study |
Plasmids | |||
 pBKJ236 |  |  | [27] |
 pKT25-zip |  |  | Euromedex |
 pUT18C-zip |  |  | Euromedex |
 pKT25 |  |  | Euromedex |
 pUT18C |  |  | Euromedex |
 pDPV392 |  | pBKJ236::∆ypeB | [20] |
 pDPV424 |  | pBKJ236::∆sleB ypeB1–446-His6 | [20] |
 pDPV448 |  | pBKJ236::∆sleB ypeB∆25–203-His6 | [20] |
 pDPV476 |  | pBKJ236::∆sleB ypeBK437C-His6 | This study |
 pDPV477 |  | pBKJ236::∆sleB ypeBS358C-His6 | This study |
 pDPV478 |  | pBKJ236::∆sleB ypeBT328C-His6 | This study |
 pDPV479 |  | pBKJ236::∆sleB ypeBM282C-His6 | This study |
 pDPV480 |  | pBKJ236::∆sleB ypeBY339C-His6 | This study |
 pDPV481 |  | pBKJ236::∆sleB ypeBE314C-His6 | This study |
 pDPV482 |  | pBKJ236::∆sleB ypeBV355C-His6 | This study |
 pDPV483 |  | pBKJ236::∆sleB ypeBV324C-His6 | This study |
 pDPV484 |  | pBKJ236::∆sleB ypeBT420C-His6 | This study |
 pDPV485 |  | pBKJ236::∆sleB ypeBA324C-His6 | This study |
 pDPV486 |  | pBKJ236::∆sleB ypeBV435C-His6 | This study |
 pDPV487 |  | pBKJ236::∆sleB ypeBQ438C-His6 | This study |
 pDPV488 |  | pBKJ236::∆sleB ypeB∆25–203 K437C-His6 | This study |
 pDPV489 |  | pBKJ236::∆sleB ypeB∆25–203 S358C-His6 | This study |
 pDPV490 |  | pUT18C::ypeB21–446 | This study |
 pDPV491 |  | pUT18C::ypeB21–202 | This study |
 pDPV492 |  | pUT18C::ypeB203–446 | This study |
 pDPV493 |  | pUT18C::sleB125–253 | This study |
 pDPV494 |  | pKT25::ypeB21–446 | This study |
 pDPV495 |  | pKT25::ypeB21–202 | This study |
 pDPV496 |  | pKT25::ypeB203–446 | This study |
 pDPV497 |  | pKT25::sleB125–253 | This study |