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Table 1 Bacterial strains and plasmids

From: YpeB dimerization may be required to stabilize SleB for effective germination of Bacillus anthracis spores

StrainGenotypeConstructionaSource
B. anthracis
 Sterne 34F2pXO1+ pXO2 P. Hanna
 DPBa89ypeBpDPV392 > 34F2[20]
 DPBa127ypeB::pDPV424 (YpeB1–446-His6 ErR)pDPV424 > DPBa89[20]
 DPBa204ypeB::pDPV476 (YpeBK437C-His6 ErR)pDPV476 > DPBa89This study
 DPBa205ypeB::pDPV477 (YpeBS358C-His6 ErR)pDPV477 > DPBa89This study
 DPBa210ypeB::pDPV478 (YpeBT328C-His6 ErR)pDPV478 > DPBa89This study
 DPBa211ypeB::pDPV479 (YpeBM282C-His6 ErR)pDPV479 > DPBa89This study
 DPBa212ypeB::pDPV480 (YpeBY339C-His6 ErR)pDPV480 > DPBa89This study
 DPBa213ypeB::pDPV481 (YpeBE314C-His6 ErR)pDPV481 > DPBa89This study
 DPBa214ypeB::pDPV482 (YpeBV355C-His6 ErR)pDPV482 > DPBa89This study
 DPBa215ypeB::pDPV483 (YpeBV324C-His6 ErR)pDPV483 > DPBa89This study
 DPBa216ypeB::pDPV484 (YpeBT420C-His6 ErR)pDPV484 > DPBa89This study
 DPBa217ypeB::pDPV485 (YpeBA342C-His6 ErR)pDPV485 > DPBa89This study
 DPBa218ypeB::pDPV486 (YpeBV435C-His6 ErR)pDPV486 > DPBa89This study
 DPBa219ypeB::pDPV487 (YpeBQ438C-His6 ErR)pDPV487 > DPBa89This study
 DPBa220ypeB::pDPV488 (YpeB∆25–203 K437C-His6 ErR)pDPV488 > DPBa89This study
 DPBa221pDPV488 (YpeB∆25–203 K437C-His6 ErR)pDPV488 > 34F2This study
 DPBa222ypeB::pDPV489 (YpeB∆25–203 S358C-His6 ErR)pDPV489 > DPBa89This study
 DPBa223pDPV489 (YpeB∆25–203 S358C-His6 ErR)pDPV489 > 34F2This study
E.coli
 BTH101  Euromedex
 DPVE545pKT25-zip + pUT18C-zippKT25-zip + pUT18C-zip > BTH101Euromedex
 DPVE546pKT25 + pUT18C This study
 DPVE547p25-YpeB21–446 + p18-YpeB21–446pDPV494 + pDPV490 > BTH101This study
 DPVE548p25-YpeB21–446 + p18-YpeB21–202pDPV494 + pDPV491 > BTH101This study
 DPVE549p25-YpeB21–446 + p18-YpeB203–446pDPV494 + pDPV492 > BTH101This study
 DPVE550p25-YpeB21–446 + p18-SleB125–253pDPV494 + pDPV493 > BTH101This study
 DPVE551p25-YpeB21–202 + p18-YpeB21–446pDPV495 + pDPV490 > BTH101This study
 DPVE552p25-YpeB21–202 + p18-YpeB21–202pDPV495 + pDPV491 > BTH101This study
 DPVE553p25-YpeB21–202 + p18-YpeB203–446pDPV495 + pDPV492 > BTH101This study
 DPVE554p25-YpeB21–202 + p18-SleB125–253pDPV495 + pDPV493 > BTH101This study
 DPVE555p25-YpeB203–446 + p18-YpeB21–446pDPV496 + pDPV490 > BTH101This study
 DPVE556p25-YpeB203–446 + p18-YpeB21–202pDPV496 + pDPV491 > BTH101This study
 DPVE557p25-YpeB203–446 + p18-YpeB203–446pDPV496 + pDPV492 > BTH101This study
 DPVE558p25-YpeB203–446 + p18-SleB125–253pDPV496 + pDPV493 > BTH101This study
 DPVE559p25-SleB125–253 + p18-YpeB21–446pDPV497 + pDPV490 > BTH101This study
 DPVE560p25-SleB125–253 + p18-YpeB21–202pDPV497 + pDPV491 > BTH101This study
 DPVE561p25-SleB125–253 + p18-YpeB203–446pDPV497 + pDPV492 > BTH101This study
 DPVE562p25-SleB125–253 + p18-SleB125–253pDPV497 + pDPV493 > BTH101This study
Plasmids
 pBKJ236  [27]
 pKT25-zip  Euromedex
 pUT18C-zip  Euromedex
 pKT25  Euromedex
 pUT18C  Euromedex
 pDPV392 pBKJ236::∆ypeB[20]
 pDPV424 pBKJ236::∆sleB ypeB1–446-His6[20]
 pDPV448 pBKJ236::∆sleB ypeB25–203-His6[20]
 pDPV476 pBKJ236::∆sleB ypeBK437C-His6This study
 pDPV477 pBKJ236::∆sleB ypeBS358C-His6This study
 pDPV478 pBKJ236::∆sleB ypeBT328C-His6This study
 pDPV479 pBKJ236::∆sleB ypeBM282C-His6This study
 pDPV480 pBKJ236::∆sleB ypeBY339C-His6This study
 pDPV481 pBKJ236::∆sleB ypeBE314C-His6This study
 pDPV482 pBKJ236::∆sleB ypeBV355C-His6This study
 pDPV483 pBKJ236::∆sleB ypeBV324C-His6This study
 pDPV484 pBKJ236::∆sleB ypeBT420C-His6This study
 pDPV485 pBKJ236::∆sleB ypeBA324C-His6This study
 pDPV486 pBKJ236::∆sleB ypeBV435C-His6This study
 pDPV487 pBKJ236::∆sleB ypeBQ438C-His6This study
 pDPV488 pBKJ236::∆sleB ypeB∆25–203 K437C-His6This study
 pDPV489 pBKJ236::∆sleB ypeB∆25–203 S358C-His6This study
 pDPV490 pUT18C::ypeB21–446This study
 pDPV491 pUT18C::ypeB21–202This study
 pDPV492 pUT18C::ypeB203–446This study
 pDPV493 pUT18C::sleB125–253This study
 pDPV494 pKT25::ypeB21–446This study
 pDPV495 pKT25::ypeB21–202This study
 pDPV496 pKT25::ypeB203–446This study
 pDPV497 pKT25::sleB125–253This study
  1. a > indicates transformation of the indicated plasmid into the indicated strain