Fig. 4

YpeB and SleB interaction detection by two-hybrid analysis. Individual domains of YpeB and SleB were inserted into both pUT18C and pKT25 creating N-terminal fusions to domains of adenylate cyclase. Plasmids were then co-transformed and screened for protein-protein interactions. Red colonies (+) indicate positive interactions. YpeB^N demonstrates strong self-association, while both YpeB^C and YpeB^N appear to interact with YpeB^Full. No interactions of YpeB domains with SleB^Cat were detected, but SleB^Cat was found to interact with itself. Images are representative of three biological replicates