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Fig. 4 | BMC Microbiology

Fig. 4

From: YpeB dimerization may be required to stabilize SleB for effective germination of Bacillus anthracis spores

Fig. 4

YpeB and SleB interaction detection by two-hybrid analysis. Individual domains of YpeB and SleB were inserted into both pUT18C and pKT25 creating N-terminal fusions to domains of adenylate cyclase. Plasmids were then co-transformed and screened for protein-protein interactions. Red colonies (+) indicate positive interactions. YpeBN demonstrates strong self-association, while both YpeBC and YpeBN appear to interact with YpeBFull. No interactions of YpeB domains with SleBCat were detected, but SleBCat was found to interact with itself. Images are representative of three biological replicates

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