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Fig. 2 | BMC Microbiology

Fig. 2

From: YpeB dimerization may be required to stabilize SleB for effective germination of Bacillus anthracis spores

Fig. 2

YpeB∆25–203-Cys can be cross-linked in spores. Ten optical density units of decoated dormant spores were cross-linked with APB as described previously. Cross-linked spores were lyophilized and mechanically broken, and proteins were extracted. Whole spore lysates were then visualized via western blot using anti-YpeB antibodies [20]. The positions of protein size markers (lane 1) are indicated on the left. YpeB monomer is indicated by the arrow at 50 kDa. YpeB∆25–203 monomer is indicated at roughly ~ 30 kDa. YpeB multimers are visualized in YpeBK437C (lane 6) migrating at 100 and 150 kDa. YpeB∆25–203 multimers are indicated in YpeB∆25-203K437C (lane 8) migrating ~ 60 kDa. A putative YpeB-YpeB∆25–203 heterodimer (lane 10) is indicated at ~ 80 kDa, in addition to homodimers identified in previous lanes

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