Detection of pathogenic Escherichia coli on potentially contaminated beef carcasses using cassette PCR and conventional PCR

Table 6 Primer sequences for STEC and O-antigen amplifications

Primer Name	Sequence	Length (bp)	Reference
O157-F	TCG TGA CAA CCA TTC CAC CTT	123	This work
O157-R	GCG CTG AAG CCT TTG GTT CT
eae-F	CAT TGA TCA GGA TTT TTC TGG TGA TA	102	[22, 23]
eae-R	CTC ATG CGG AAA TAG CCG TTA
stx1-F	GTG GCA AGA GCG ATG TTA CGG TTT G	182	[24]
stx1-R	ATG ATA GTC AGG CAG GAC GCT ACT C
stx2-F	ACG AGG GCT TGA TGT CTA TCA GGC G	200	[24]
stx2-R	GCG ACA CGT TGC AGA GTG GTA TAA C
O26-F	GTA TCG CTG AAA TTA GAA GCG C	158	[23, 25]
O26-R	AGT TGA AAC ACC CGT AAT GGC
O45-F	TAT GAC AGG CAC ATG GAT CTG TGG	132	[26]
O45-R	CAC AAC GCA ACG AAA GTC CC		[27]
O103-F	AAT TGC TCT ATG CGC TCT TCC	136	This work
O103-R	GCC CAC CAT AGA TAA CAA CGA
O111-F	GGA ATA ATC GAC CGG CCA AA	199	This work
O111-R	TAA TGT GTT GCC TCG CCT TC
O121-F	TGT TGG CTA GTG GCA TTC TGA	212	This work
O121-R	TTC TGC ATC ACC AGT CCA GA		[26]
O145-F	AAA CTG GGA TTG GAC GTG G	135	[25]
O145-R	CCC AAA ACT TCT AGG CCC G

ISSN: 1471-2180