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Table 6 Primer sequences for STEC and O-antigen amplifications

From: Detection of pathogenic Escherichia coli on potentially contaminated beef carcasses using cassette PCR and conventional PCR

Primer NameSequenceLength (bp)Reference
O157-FTCG TGA CAA CCA TTC CAC CTT123This work
O157-RGCG CTG AAG CCT TTG GTT CT  
eae-FCAT TGA TCA GGA TTT TTC TGG TGA TA102[22, 23]
eae-RCTC ATG CGG AAA TAG CCG TTA  
stx1-FGTG GCA AGA GCG ATG TTA CGG TTT G182[24]
stx1-RATG ATA GTC AGG CAG GAC GCT ACT C  
stx2-FACG AGG GCT TGA TGT CTA TCA GGC G200[24]
stx2-RGCG ACA CGT TGC AGA GTG GTA TAA C  
O26-FGTA TCG CTG AAA TTA GAA GCG C158[23, 25]
O26-RAGT TGA AAC ACC CGT AAT GGC  
O45-FTAT GAC AGG CAC ATG GAT CTG TGG132[26]
O45-RCAC AAC GCA ACG AAA GTC CC [27]
O103-FAAT TGC TCT ATG CGC TCT TCC136This work
O103-RGCC CAC CAT AGA TAA CAA CGA  
O111-FGGA ATA ATC GAC CGG CCA AA199This work
O111-RTAA TGT GTT GCC TCG CCT TC  
O121-FTGT TGG CTA GTG GCA TTC TGA212This work
O121-RTTC TGC ATC ACC AGT CCA GA [26]
O145-FAAA CTG GGA TTG GAC GTG G135[25]
O145-RCCC AAA ACT TCT AGG CCC G