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Fig. 4 | BMC Microbiology

Fig. 4

From: Universal membrane-labeling combined with expression of Katushka far-red fluorescent protein enables non-invasive dynamic and longitudinal quantitative 3D dual-color fluorescent imaging of multiple bacterial strains in mouse intestine

Fig. 4

Mice co-fed with colonizing and non-colonizing bacteria show differential localization 24 h after feeding. Mice were co-fed colonizing, fluorescently-labeled E. coli Nissle 1917 and the TOP10 bacteria constitutively expressing the fluorescent protein Katushka. 24 h after feeding, FMT images show a distinct localization pattern with the Nissle 1917 bacteria found within the upper digestive tract while the TOP10 bacteria are present much lower within the mice (a). Ex vivo analyses of the intestinal tract by NightOwl imaging show substantial amounts of Nissle 1917 cells present in the early ileum while the TOP10 bacteria are more confined to the terminal ileum and colon (b)

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