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Fig. 6 | BMC Microbiology

Fig. 6

From: Transcriptional responses of Xanthomonas oryzae pv. oryzae to type III secretion system inhibitor ortho-coumaric acid

Fig. 6

PXO_RS13760 was involved in the response of OCA in Xoo but had no relationship with the regulation of T3SS. a Relative mRNA levels of PXO_RS13745, PXO_RS13750 and PXO_RS13755 in wild type and PXO_RS13760 deletion mutant were measured by qRT-PCR. Xoo cells were cultured in M210 medium overnight and subcultures to XOM2 medium for 6 hours. Values represent the levels of expression compared to that of wildtype (for which the level of expression was set equal to 1.00); b Relative mRNA levels of PXO_RS13745, PXO_RS13750 and PXO_RS13755 in wild type and PXO_RS13760 deletion mutant under OCA treatment were measured by qRT-PCR. Xoo cells were cultured in M210 medium overnight and subcultures to XOM2 medium. After 1 h incubation, OCA was added to the medium at a concentration of 200 μM and incubated for 6 h. Values represent the levels of expression compared to that of wildtype without OCA treatment (for which the level of expression was set equal to 1.00); c Relative mRNA levels of hrp genes in wildtype and PXO_RS13760 deletion mutant were measured by qRT-PCR. Xoo cells were cultured in M210 medium overnight and subcultures to XOM2 medium for 6 hours. Values represent the levels of expression compared to that of wildtype (for which the level of expression was set equal to 1.00); d Relative mRNA levels of hrp genes in PXO_RS13760 deletion mutant under OCA treatment were measured by qRT-PCR. Xoo cells were cultured in M210 medium overnight and subcultures to XOM2 medium. After 1 h incubation, OCA was added to the medium at a concentration of 200 μM and incubated for 6 h. Values represent the levels of expression compared to that of bacterial cells without OCA treatment (for which the level of expression was set equal to 1.00). Asterisks indicate statistically significant differences in the expression levels (Student’s t-test). * P < 0.05; ** P < 0.01

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