Table 3 Primer sequences for real-time PCR
Gene | Primer sequence | NCBI Reference Sequence: | References |
|---|---|---|---|
NF-κB p65 | Forward 5′ - GGACAGCACCACCTACGATG - 3′ Reverse 5′ - CTGGATCACTTCAATGGCCTC - 3’ | NM_009045.4 | Present study |
I-κB | Forward 5’ - CAGGAGCCAAAACCGACAAC - 3′ Reverse 5′ - TGGTTGTCAGGTCTGCAATTTT - 3’ | NM_001306222.1 | Present study |
Fas | Forward 5’ - CCAAACGGAAATTGCAGGGG - 3′ Reverse 5′ - AAGCACCAGTTCACAGATGGA - 3’ | NM_001146708.1 | Present study |
FADD | Forward 5’ - TGCTCCACCTATCCACCAGA - 3′ Reverse 5′ - CAATGCGGAAGGCGATTGAG - 3’ | NM_010175.6 | Present study |
TRADD | Forward 5’ - GAGCTGCTGGAGTGCAACTA - 3′ Reverse 5′ - GGTCCGGGTACTTAGAGGGT - 3’ | NM_001033161.2 | Present study |
Caspase-8 | Forward 5’ - CCAGACAGAGAAGGGGCTTG - 3′ Reverse 5′ - TCACTGCCCAGTTCTTCAGC - 3’ | NM_001080126.1 | Present study |
IL-1β | Forward 5’ - TCGTGCTGTCGGACCCATAT - 3′ Reverse 5′ - GTCGTTGCTTGGTTCTCCTTGT - 3’ | NM_008361.4 | Present study |
IL-6 | Forward 5’ - GACAAAGCCAGAGTCCTTCAGA - 3′ Reverse 5′ - TGTGACTCCAGCTTATCTCTTGG - 3’ | NM_001314054.1 | Present study |
TNF-α | Forward 5’ - GCGGAGTCCGGGCAGGTCTA - 3′ Reverse 5′ - GGGGGCTGGCTCTGTGAGGA - 3’ | NM_001278601.1 | Present study |
β-actin | Forward 5’ - TGCTGTCCCTGTATGCCTCTG - 3′ Reverse 5′ - TTGATGTCACGCACGATTTCC - 3’ | NM_007393.4 | Present study |