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Table 3 Mass spectrometry identification of C. tropicalis moonlighting proteins present at the cell surface after growth under different conditions

From: Moonlighting proteins are variably exposed at the cell surfaces of Candida glabrata, Candida parapsilosis and Candida tropicalis under certain growth conditions

NCBI accession number

Protein description

defined synthetic medium (DS)

artificial saliva (AS)

vagina- simulative medium (VS)

artificial urine (AU)

anaerobic conditions (AN)

gi|255,727,881 (XP_002548866)

enolase 1 (Eno1) CTRG_03163 [Candida tropicalis MYA-3404]

0.08812

0.27053**

–

0.32363**

0.15934 ns

gi|255,729,208 (XP_002549529)

pyruvate decarboxylase (Pdc11) CTRG_03826 [Candida tropicalis MYA-3404]

0.04341

–

–

–

0.03722 ns

gi|255,732,890 (XP_002551368)

glyceraldehyde-3-phosphate dehydrogenase (Tdh3) CTRG_05666 [Candida tropicalisMYA-3404]

0.09736

–

0.57733****

–

0.09534 ns

  1. Cell surface shaving of C. tropicalis cultures with trypsin and additional digestion of the obtained proteins for 24 h was performed. The resulting peptides were then analyzed using the Dionex Ultimate 3000 UHPLC system coupled to an HCTUltra ETDII mass spectrometer. The obtained lists of peaks were searched against the NCBI protein database using an in-house Mascot server. The normalized abundance factors (NSAFs) were calculated for each of the tested growth conditions and the statistical significance with respect to the defined synthetic medium is indicated as follows:**p from 0.001 to 0.01; ****p < 0.0001; and ns, not significant by Student t-test
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