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Table 2 Mass spectrometry identification of C. parapsilosis moonlighting proteins present at the cell surface under different growth conditions

From: Moonlighting proteins are variably exposed at the cell surfaces of Candida glabrata, Candida parapsilosis and Candida tropicalis under certain growth conditions

NCBI accession number Protein description defined synthetic medium (DS) artificial saliva (AS) vagina-simulative medium (VS) artificial urine (AU) anaerobic conditions (AN)
gi|354,546,348
(CCE43078)
hypothetical protein CPAR2_207210 [Candida parapsilosis]
enolase (Eno1)
0.07214 0.09507 ns 0.06579 ns
gi|354,545,590
(CCE42318)
hypothetical protein CPAR2_808670 [Candida parapsilosis]
glyceraldehyde-3-phosphate dehydrogenase (Tdh3)
0.07167 0.11546 ns 0.07485 ns
gi|354,545,888
(CCE42617)
hypothetical protein CPAR2_202600 [Candida parapsilosis]
transaldolase (Tal1)
0.06634 0.08495 ns 0.02001**
gi|354,547,143
(CCE43876)
hypothetical protein CPAR2_501020 [Candida parapsilosis]
pyruvate decarboxylase (Pdc11)
0.01985 0.03279*** 0.02973 ns
gi|354,543,158
(CCE39876)
hypothetical protein CPAR2_602950 [Candida parapsilosis]
phosphoglycerate kinase (Pgk1)
0.12601 0.08621
gi|354,543,177
(CCE39895)
hypothetical protein CPAR2_603140 [Candida parapsilosis]
putative ketol-acid reductoisomerase (Ilv5)
0.01782
gi|354,543,405
(CCE40124)
hypothetical protein CPAR2_101620 [Candida parapsilosis]
acetyl-coA hydrolase (Ach1)
0.01021
gi|354,545,521
(CCE42249)
hypothetical protein CPAR2_807980 [Candida parapsilosis]
triosephosphate isomerase (Tpi)
0.04040
gi|354,545,980
(CCE42709)
hypothetical protein CPAR2_203520 [Candida parapsilosis]
6-phosphogluconate dehydrogenase (Gnd1)
0.01057
gi|354,546,116
(CCE42845)
hypothetical protein CPAR2_204880 [Candida parapsilosis]
phosphoglucose isomerase (Pgi1)
0.01127
gi|354,546,805
(CCE43537)
hypothetical protein CPAR2_211810 [Candida parapsilosis]
phosphoglycerate mutase (Gpm1)
0.07052
gi|354,546,845
(CCE43577)
hypothetical protein CPAR2_212210 [Candida parapsilosis]
NAD-aldehyde dehydrogenase (Ald5)
0.23428  
gi|354,547,299
(CCE44033)
hypothetical protein CPAR2_502580 [Candida parapsilosis]
alcohol dehydrogenase (Adh1)
0.13296 0.08365 0.13991
gi|354,547,586
(CCE44321)
hypothetical protein CPAR2_401230 [Candida parapsilosis]
fructose-bisphosphate aldolase (Fba1)
0.04268
  1. Cell surface shaving of C. parapsilosis cultures with trypsin and additional digestion of the obtained proteins for 24 h was performed. The resulting peptides were then analyzed using the Dionex Ultimate 3000 UHPLC system coupled to an HCTUltra ETDII mass spectrometer. The obtained lists of peaks were searched against the NCBI protein database using an in-house Mascot server. The normalized abundance factors (NSAFs) were calculated for each of the tested growth conditions and the statistical significance with respect to the defined synthetic medium is indicated as follows: **p from 0.001 to 0.01, ***p from 0.0001 to 0.001; and ns, not significant by Student t-test. The arrows indicate that the protein was only identified from cultures grown in AS, VS, AU or AN and not in the synthetic medium