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Table 1 Mass spectrometry identification of C. glabrata moonlighting proteins present at the cell surface under different growth conditions

From: Moonlighting proteins are variably exposed at the cell surfaces of Candida glabrata, Candida parapsilosis and Candida tropicalis under certain growth conditions

NCBI accession number Protein description defined synthetic medium (DS) artificial saliva (AS) vagina-simulative medium (VS) artificial urine (AU) anaerobic conditions (AN)
gi|50,288,681
(XP_446770)
uncharacterized protein CAGL0G09383g [Candida glabrata]
glyceraldehyde-3-phosphate dehydrogenase 3 (Tdh3)
0.10203 0.06654 ns 0.26374* 0.10739 ns 0.08619 ns
gi|50,289,857
(XP_447360)
uncharacterized protein CAGL0I02486g [Candida glabrata]
enolase I (Eno1)
0.04892 0.08869* 0.18915 ns 0.08158* 0.12634*
gi|50,293,403
(XP_449113)
uncharacterized protein CAGL0L07722g [Candida glabrata]
phosphoglycerate kinase (Pgk1)
0.03511 0.03084 ns 0.03684 ns 0.05591 ns
gi|50,287,073
(XP_445966)
uncharacterized protein CAGL0E06358g [Candida glabrata]
phosphoglycerate mutase 1 (Gpm1)
0.02871 0.02756 ns
gi|50,285,355
(XP_445106)
uncharacterized protein CAGL0B03069g [Candida glabrata]
transaldolase (Tal1)
0.01863 0.03913* 0.01621 ns
gi|50,294,908
(XP_449865)
uncharacterized protein CAGL0M12034g [Candida glabrata]
pyruvate kinase (Cdc19)
0.01426 0.05353**** 0.03152*
gi|50,292,893
(XP_448879)
uncharacterized protein CAGL0L02497g [Candida glabrata]
fructose-bisphosphate aldolase (Fba1)
0.01149 0.04445*
gi|50,295,024
(XP_449923)
uncharacterized protein CAGL0M13343g [Candida glabrata]
6-phosphogluconate dehydrogenase (Gnd1)
0.00730 0.01415 ns  
gi|25,992,752
(AAN77243)
pyruvate decarboxylase (Pdc11) [Candida glabrata] 0.05196 0.08659 0.02002
gi|50,286,085
(XP_445471)
uncharacterized protein CAGL0D01298g [Candida glabrata]
transketolase (Tkl1)
0.01866 0.01398
gi|50,289,205
(XP_447033)
uncharacterized protein CAGL0H05445g [Candida glabrata]
glucose-6-phosphate isomerase (Pgi1)
0.02312
gi|50,289,307
(XP_447084)
uncharacterized protein CAGL0H06633g [Candida glabrata]
phosphoenolpyruvate carboxykinase (Pck1)
0.03884
gi|50,289,459
(XP_447161)
uncharacterized protein CAGL0H08327g [Candida glabrata]
triosephosphate isomerase (Tpi1)
0.07053 0.03771
gi|50,289,591
(XP_447227)
uncharacterized protein CAGL0H09878g [Candida glabrata]
inorganic pirophosphatase (Ipp1)
0.03773
gi|50,290,317
(XP_447590)
uncharacterized protein CAGL0I07843g [Candida glabrata]
alcohol dehydrogenase I (Adh1)
0.04322 0.03855
gi|50,291,871
(XP_448368)
uncharacterized protein CAGL0K03289g [Candida glabrata]
glucose-6-phosphate 1-epimerase (Gpe1)
0.00787
gi|50,292,233
(XP_448549)
uncharacterized protein CAGL0K07546g [Candida glabrata]
probable phosphoglycerate mutase (Pmu1)
0.31504
gi|50,293,881
(XP_449352)
uncharacterized protein CAGL0M00176g [Candida glabrata]
branched-chain-amino-acid aminotransferase (Twt2)
0.01708
gi|50,294,878
(XP_449850)
uncharacterized protein CAGL0M11704g [Candida glabrata]
alkyl hydroperoxide reductase (Ahp1)
0.03156
  1. Cell surface shaving of C. glabrata cultures with trypsin and the additional digestion of the obtained proteins for 24 h was performed. The resulting peptides were analyzed using the Dionex Ultimate 3000 UHPLC system coupled to an HCTUltra ETDII mass spectrometer. The obtained lists of peaks were searched against the NCBI protein database using an in-house Mascot server. The normalized abundance factors (NSAFs) were calculated for each of the tested growth conditions and the statistical significance with respect to the defined synthetic medium is indicated as follows: *p values from 0.01 to 0.05; ****p < 0.0001; ns, not significant by Student t-test. The arrows indicate that the protein was only identified from cultures grown in AS, VS, AU or AN and not in the synthetic medium