Fig. 4

Semiquantitative PCR of Asaia and b, d, f, and h image analyses of the agarose gel in ‘a, c, e, and g’, for quantifying the abundance of Asaia in different long- and short-winged adult BPH samples. The actin gene served as the internal control. Field-LWBM: field-collected long-winged adult male BPH; Field-LWBF: field-collected long-winged adult female BPH; LWBM-HHR: long-winged adult male BPH collected from the laboratory “HHZ” (susceptible) rice variety; LWBF-HHR: long-winged adult female BPH collected from laboratory HHZ; LWBM-IR36: long-winged adult male BPH collected from the “IR36” (resistant) rice variety; LWBF-IR36: long-winged adult female BPH collected from IR36; Field-SWBM: field-collected short-winged adult male BPH; Field-SWBF: field-collected short-winged adult female BPH; SWBM-HHR: short-winged adult male BPH collected from the laboratory “HHZ” (susceptible) rice variety; SWBF-HHR: short-winged adult female BPH collected from laboratory HHZ; SWBM-IR36: short-winged adult male BPH collected from the “IR36” (resistant) rice variety; SWBF-IR36: short-winged adult female BPH collected from IR36. ImageJ analyses of the agarose gel in ‘i’, for quantifying abundance of Asaia in BPH-egg and BPH-egg infected rice samples