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Fig. 8 | BMC Microbiology

Fig. 8

From: PgFur participates differentially in expression of virulence factors in more virulent A7436 and less virulent ATCC 33277 Porphyromonas gingivalis strains

Fig. 8

Expression of P. gingivalis hmuY gene. Determination of hmuY transcript was analyzed using RT-qPCR (a) and HmuY protein using Western blotting with anti-HmuY antibodies (b). Bacteria were grown under high- (Hm) or low-iron/heme (DIP) conditions in a liquid culture medium. Relative gene expression was determined in pgfur mutant (TO6 and TO16) strains versus wild-type (A7436 and ATCC 33277) strains. WC, whole bacterial culture; CM, culture medium; OMV, outer-membrane vesicles. *P < 0.05, **P < 0.01, ***P < 0.001

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