Fig. 7From: PgFur participates differentially in expression of virulence factors in more virulent A7436 and less virulent ATCC 33277 Porphyromonas gingivalis strainsAnalysis of expression of selected glycosyltransferase gene and glycosylation pattern of glycoconjugates produced by P. gingivalis wild-type (A7436 and ATCC 33277) and pgfur mutant (TO6 and TO16) strains. Bacteria were grown under high- (Hm) or low-iron/heme (DIP) conditions in liquid culture medium. a Relative gene expression was determined at the indicated time points in pgfur mutant (TO6 and TO16) strains versus wild-type (A7436 and ATCC 33277) strains by RT-qPCR. b Whole bacterial lysates were separated by SDS-PAGE, transferred onto nitrocellulose membranes and probed with selected lectins. MAL II, sialic acid; AAL, fructose linked to N-acetylglucosamine with α-1,6 linkage or to N-acetyllactosamine with α-1,3 linkage, arabinose; SNA, sialic acid linked to the terminal galactose with α-2,6 or α-2,3 linkage; RCA 120, galactose and N-acetylgalactosamine; JACALIN, galactose linked to N-acetylgalactosamine with β-1,3 linkage. Equal loading of proteins was visualized in SDS-PAGE gels by staining with Coomassie Brilliant Blue G-250 (loading control). *P < 0.05, **P < 0.01, ***P < 0.001Back to article page