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Fig. 7 | BMC Microbiology

Fig. 7

From: PgFur participates differentially in expression of virulence factors in more virulent A7436 and less virulent ATCC 33277 Porphyromonas gingivalis strains

Fig. 7

Analysis of expression of selected glycosyltransferase gene and glycosylation pattern of glycoconjugates produced by P. gingivalis wild-type (A7436 and ATCC 33277) and pgfur mutant (TO6 and TO16) strains. Bacteria were grown under high- (Hm) or low-iron/heme (DIP) conditions in liquid culture medium. a Relative gene expression was determined at the indicated time points in pgfur mutant (TO6 and TO16) strains versus wild-type (A7436 and ATCC 33277) strains by RT-qPCR. b Whole bacterial lysates were separated by SDS-PAGE, transferred onto nitrocellulose membranes and probed with selected lectins. MAL II, sialic acid; AAL, fructose linked to N-acetylglucosamine with α-1,6 linkage or to N-acetyllactosamine with α-1,3 linkage, arabinose; SNA, sialic acid linked to the terminal galactose with α-2,6 or α-2,3 linkage; RCA 120, galactose and N-acetylgalactosamine; JACALIN, galactose linked to N-acetylgalactosamine with β-1,3 linkage. Equal loading of proteins was visualized in SDS-PAGE gels by staining with Coomassie Brilliant Blue G-250 (loading control). *P < 0.05, **P < 0.01, ***P < 0.001

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