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Table 1 Designed primers and fragment Tm of the amplified fragments for multiplex real-time PCR

From: Rapid detection and molecular survey of blaVIM, blaIMP and blaNDM genes among clinical isolates of Acinetobacter baumannii using new multiplex real-time PCR and melting curve analysis

Resistance genes

Primer (5′-3′)

Fragment length (bp)

Fragment melting Temprature (°C)

IMP

F: TTGACACTCCATTTACTGCTA

R: TCATTTGTTAATTCAGATGCATA

172

83.5

VIM

F: GAGTTGCTTTTGATTGATACAG

R: TCGATGAGAGTCCTTCTAGA

247

93.3

NDM

F: AACACAGCCTGACTTTCG

R: TGATATTGTCACTGGTGTGG

111

89.3